Fentanyl induces autophagy via activation of the ROS/MAPK pathway and reduces the sensitivity of cisplatin in lung cancer cells

  • Authors:
    • Jiaqi Yao
    • Chi Ma
    • Wei Gao
    • Jinxiao Liang
    • Chang Liu
    • Hongfang Yang
    • Qiu Yan
    • Qingping Wen
  • View Affiliations

  • Published online on: October 19, 2016     https://doi.org/10.3892/or.2016.5183
  • Pages: 3363-3370
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Cancer pain is the most common complication of lung carcinoma. Opioid agonist fentanyl is widely used for relieving pain in cancer patients, and cisplatin (DDP)‑based chemotherapy is commonly used for the treatment of advanced lung cancer; these two drugs are always used together in lung carcinoma patients. However, the mechanisms and related biological pathways by which fentanyl influences cisplatin sensitivity are relatively poorly reported. Here, we found that fentanyl reduces the sensitivity of cisplatin in human lung cancer cells and induces autophagy. Fentanyl induced reactive oxygen species (ROS) generation and JNK activation. N-acetyl‑L‑cysteine is a ROS scavenger and antioxidant, and the inhibition of JNK with SP600125 prevented fentanyl‑induced autophagy. We also found that 3-methyladenine (3-MA; an autophagy inhibitor) increased the sensitivity of DDP and weakened the inhibition of fentanyl. In conclusion, fentanyl reduces the sensitivity of cisplatin in lung cancer cells through the ROS-JNK-autophagy pathway, whereas the autophagy inhibitor 3-MA may weaken this effect.
View Figures
View References

Related Articles

Journal Cover

December-2016
Volume 36 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Yao J, Ma C, Gao W, Liang J, Liu C, Yang H, Yan Q and Wen Q: Fentanyl induces autophagy via activation of the ROS/MAPK pathway and reduces the sensitivity of cisplatin in lung cancer cells. Oncol Rep 36: 3363-3370, 2016
APA
Yao, J., Ma, C., Gao, W., Liang, J., Liu, C., Yang, H. ... Wen, Q. (2016). Fentanyl induces autophagy via activation of the ROS/MAPK pathway and reduces the sensitivity of cisplatin in lung cancer cells. Oncology Reports, 36, 3363-3370. https://doi.org/10.3892/or.2016.5183
MLA
Yao, J., Ma, C., Gao, W., Liang, J., Liu, C., Yang, H., Yan, Q., Wen, Q."Fentanyl induces autophagy via activation of the ROS/MAPK pathway and reduces the sensitivity of cisplatin in lung cancer cells". Oncology Reports 36.6 (2016): 3363-3370.
Chicago
Yao, J., Ma, C., Gao, W., Liang, J., Liu, C., Yang, H., Yan, Q., Wen, Q."Fentanyl induces autophagy via activation of the ROS/MAPK pathway and reduces the sensitivity of cisplatin in lung cancer cells". Oncology Reports 36, no. 6 (2016): 3363-3370. https://doi.org/10.3892/or.2016.5183