Protective effects of quercetin on UVB irradiation‑induced cytotoxicity through ROS clearance in keratinocyte cells

  • Authors:
    • Xianbing Zhu
    • Ning Li
    • Yiling Wang
    • Li Ding
    • Houjie Chen
    • Yehui Yu
    • Xiaojun Shi
  • View Affiliations

  • Published online on: November 4, 2016     https://doi.org/10.3892/or.2016.5217
  • Pages: 209-218
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Abstract

Human skin is the body's largest organ that protects against diverse environmental injuries. However, ultraviolet (UV) radiation, which induces a transient increase in the intracellular level of reactive oxygen species (ROS) and leads to a variety of injuries and various skin diseases, has deleterious effects on living organisms. Quercetin is a naturally occurring compound with strong antioxidant action and can successfully scavenge free radicals. In the present study, we investigated the effects and the mechanism of quercetin on UVB‑induced cytotoxicity in keratinocyte (HaCaT) cells. The results of this study showed that quercetin (20 μM) significantly blocked UVB irradiation (15 mJ/cm2)‑induced intracellular ROS generation. In addition, the ROS clearing ability of quercetin prevented cell membrane and mitochondria from ROS attack and inhibited cell membrane fluidity decrease and mitochondrial membrane depolarization. Moreover, the outflow of cytochrome c and apoptosis were markedly inhibited. These results suggest that the protective effect of quercetin against UVB irradiation‑induced toxicity is mainly mediated by the ROS scavenging ability. Thus, quercetin is a potential agent against UVB irradiation‑induced skin damage.
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January-2017
Volume 37 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Zhu X, Li N, Wang Y, Ding L, Chen H, Yu Y and Shi X: Protective effects of quercetin on UVB irradiation‑induced cytotoxicity through ROS clearance in keratinocyte cells. Oncol Rep 37: 209-218, 2017
APA
Zhu, X., Li, N., Wang, Y., Ding, L., Chen, H., Yu, Y., & Shi, X. (2017). Protective effects of quercetin on UVB irradiation‑induced cytotoxicity through ROS clearance in keratinocyte cells. Oncology Reports, 37, 209-218. https://doi.org/10.3892/or.2016.5217
MLA
Zhu, X., Li, N., Wang, Y., Ding, L., Chen, H., Yu, Y., Shi, X."Protective effects of quercetin on UVB irradiation‑induced cytotoxicity through ROS clearance in keratinocyte cells". Oncology Reports 37.1 (2017): 209-218.
Chicago
Zhu, X., Li, N., Wang, Y., Ding, L., Chen, H., Yu, Y., Shi, X."Protective effects of quercetin on UVB irradiation‑induced cytotoxicity through ROS clearance in keratinocyte cells". Oncology Reports 37, no. 1 (2017): 209-218. https://doi.org/10.3892/or.2016.5217