miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer

  • Authors:
    • Guanli Huang
    • Xiangjian Chen
    • Yefeng Cai
    • Xiaobo Wang
    • Chungen Xing
  • View Affiliations

  • Published online on: November 28, 2016     https://doi.org/10.3892/or.2016.5278
  • Pages: 571-578
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Abstract

MicroRNAs (miRNAs) are small, non-coding RNAs that play important roles in cancer processes. Although miR-20a has been reported to be altered in a range of cancers, the role of miR-20a in colorectal cancer is not fully characterized, and the relationship between miR-20a dysregulation and the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) sensitivity is not defined. In the present study, we demonstrated significant upregulation of miR-20a in the serum of colorectal cancer patients, tumor tissues and cell lines by quantitative RT-PCR analysis. Furthermore, we found that the TRAIL-induced apoptosis was associated with the expression level of miR-20a in colorectal cancer. The knockdown of miR-20a by inhibitors increased the antitumor effect of TRAIL via caspase-8 dependent pathway. BID, which is a pro-apoptotic member of the Bcl-2 family, was found to be directly regulated by miR-20a in SW480 cells. The knockdown of miR-20a inhibited the translocation of tBID to the mitochondria, which induced the mitochondrial pathway of apoptosis. Notably, we found that the knockdown of miR-20a also reversed the resistance of TRAIL in established TRAIL-resistant SW480 cells by tBID-mitochondria pathway. Therefore, our results suggest that miR-20a acts as a tumor promoter in colorectal cancer, and the understanding of the miR-20a might be a potential therapeutic target for colorectal cancer.
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January-2017
Volume 37 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Huang G, Chen X, Cai Y, Wang X and Xing C: miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer. Oncol Rep 37: 571-578, 2017
APA
Huang, G., Chen, X., Cai, Y., Wang, X., & Xing, C. (2017). miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer. Oncology Reports, 37, 571-578. https://doi.org/10.3892/or.2016.5278
MLA
Huang, G., Chen, X., Cai, Y., Wang, X., Xing, C."miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer". Oncology Reports 37.1 (2017): 571-578.
Chicago
Huang, G., Chen, X., Cai, Y., Wang, X., Xing, C."miR-20a-directed regulation of BID is associated with the TRAIL sensitivity in colorectal cancer". Oncology Reports 37, no. 1 (2017): 571-578. https://doi.org/10.3892/or.2016.5278