Open Access

ELF5 in epithelial ovarian carcinoma tissues and biological behavior in ovarian carcinoma cells

  • Authors:
    • Hongchao Yan
    • Linglin Qiu
    • Xiaolei Xie
    • He Yang
    • Yongli Liu
    • Xiaoman Lin
    • Hongxiang Huang
  • View Affiliations

  • Published online on: February 2, 2017     https://doi.org/10.3892/or.2017.5418
  • Pages: 1412-1418
  • Copyright: © Yan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The expression of E74-like factor 5 (ELF5) in epithelial ovarian carcinoma tissues and its effects on biological behavior in ovarian carcinoma cells were assessed in search for a new approach for gene treatment of epithelial ovarian carcinoma. RT-PCR technology was applied to detect the expression of ELF5 mRNA in epithelial ovarian carcinoma (n=49), borderline ovarian epithelial tumor (n=19), benign ovarian epithelial tumor (n=31) and normal ovarian tissues (n=40). Then, we transfected recombinant plasmid pcDNA3.1‑ELF5+EGFP into human ovarian carcinoma SKOV3 cells (recombinant plasmid group) in vitro and screened out stably transfected cells to conduct multiplication culture. Western blot analysis was performed to detect the expression of ELF5 protein in the different groups. Flow cytometry was employed to detect cell apoptosis and cycles. ELF5 mRNA in epithelial ovarian carcinoma and borderline ovarian epithelial tumor tissues were significantly lower (P<0.05) than those in benign ovarian epithelial tumor and normal ovarian tissues. ELF5 protein expression in the cells of recombinant plasmid group was significantly higher compared with empty plasmid and blank control groups. The capacity of cell reproductive recombinant plasmid group at each time point decreased (P<0.05). Flow cytometry detection showed that 67.03% of cells in recombinant plasmid group was blocked in G0/G1 phase (P<0.05), compared with empty plasmid group (37.17%) and blank control group (38.24%). Apoptotic rate of recombinant plasmid group was significantly lower (31.4±1.9%; P<0.05), compared with that of empty plasmid group (9.1±2.2%) and blank control group (8.7±1.5%), and the differences were statistically significant. In conclusion, ELF5 interfered with cell cycle of human ovarian carcinoma SKOV3 cells and promoted apoptosis of human ovarian carcinoma SKOV3 cells inhibiting their growth and invasive capacity; and thus providing a new approach to gene treatment of ovarian carcinoma.
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March-2017
Volume 37 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Yan H, Qiu L, Xie X, Yang H, Liu Y, Lin X and Huang H: ELF5 in epithelial ovarian carcinoma tissues and biological behavior in ovarian carcinoma cells. Oncol Rep 37: 1412-1418, 2017
APA
Yan, H., Qiu, L., Xie, X., Yang, H., Liu, Y., Lin, X., & Huang, H. (2017). ELF5 in epithelial ovarian carcinoma tissues and biological behavior in ovarian carcinoma cells. Oncology Reports, 37, 1412-1418. https://doi.org/10.3892/or.2017.5418
MLA
Yan, H., Qiu, L., Xie, X., Yang, H., Liu, Y., Lin, X., Huang, H."ELF5 in epithelial ovarian carcinoma tissues and biological behavior in ovarian carcinoma cells". Oncology Reports 37.3 (2017): 1412-1418.
Chicago
Yan, H., Qiu, L., Xie, X., Yang, H., Liu, Y., Lin, X., Huang, H."ELF5 in epithelial ovarian carcinoma tissues and biological behavior in ovarian carcinoma cells". Oncology Reports 37, no. 3 (2017): 1412-1418. https://doi.org/10.3892/or.2017.5418