Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma

  • Authors:
    • André Luis Giacometti Conceição
    • Camila Tainah Da Silva
    • Rodolfo Miglioli Badial
    • Marina Curado Valsechi
    • Bruna Stuqui
    • Jéssica Domingues Gonçalves
    • Miriam Galvonas Jasiulionis
    • Marilia De Freitas Calmon
    • Paula Rahal
  • View Affiliations

  • Published online on: January 31, 2017     https://doi.org/10.3892/or.2017.5414
  • Pages: 1487-1496
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Abstract

Clear cell renal cell carcinoma (ccRCC) is the most common histological subtype of kidney cancer. This carcinoma is histologically characterized by the presence of clear and abundant cytoplasm. In the present study, we sought to identify genes differentially expressed in ccRCC and build a molecular profile of this cancer. We selected genes described in the literature related to cellular differentiation and proliferation. We analyzed the gene and protein expression by quantitative PCR (qPCR) and immunohistochemistry, respectively, and examined possible epigenetic mechanisms that regulate their expression in ccRCC samples and cell lines. Occludin (OCLN) and growth arrest-specific 1 (GAS1) genes were underexpressed in ccRCC, and we report that miR-122 and miR-34a, respectively, may regulate their expression in this cancer. Furthermore, we showed by qPCR and immunohistochemistry that solute carrier family 2 member 1 (SLC2A1) was significantly overexpressed in ccRCC. The set of genes identified in the present study furthers our understanding of the molecular basis and development of ccRCC.
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March-2017
Volume 37 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Conceição AG, Da Silva CT, Badial RM, Valsechi MC, Stuqui B, Gonçalves JD, Jasiulionis MG, De Freitas Calmon M and Rahal P: Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma. Oncol Rep 37: 1487-1496, 2017
APA
Conceição, A.G., Da Silva, C.T., Badial, R.M., Valsechi, M.C., Stuqui, B., Gonçalves, J.D. ... Rahal, P. (2017). Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma. Oncology Reports, 37, 1487-1496. https://doi.org/10.3892/or.2017.5414
MLA
Conceição, A. G., Da Silva, C. T., Badial, R. M., Valsechi, M. C., Stuqui, B., Gonçalves, J. D., Jasiulionis, M. G., De Freitas Calmon, M., Rahal, P."Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma". Oncology Reports 37.3 (2017): 1487-1496.
Chicago
Conceição, A. G., Da Silva, C. T., Badial, R. M., Valsechi, M. C., Stuqui, B., Gonçalves, J. D., Jasiulionis, M. G., De Freitas Calmon, M., Rahal, P."Downregulation of OCLN and GAS1 in clear cell renal cell carcinoma". Oncology Reports 37, no. 3 (2017): 1487-1496. https://doi.org/10.3892/or.2017.5414