Tumor-penetrating peptide fused to a pro-apoptotic peptide facilitates effective gastric cancer therapy

  • Authors:
    • Ying Huang
    • Xihan Li
    • Huizi Sha
    • Lianru Zhang
    • Xinyu Bian
    • Xiao Han
    • Baorui Liu
  • View Affiliations

  • Published online on: February 10, 2017     https://doi.org/10.3892/or.2017.5440
  • Pages: 2063-2070
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

KLA (sequence, KLAKLAKKLAKLAK) is a peptide which leads to programmed cell death by disrupting the mitochondrial membrane. However, low penetration in tumors greatly limits its application and efficacy. To develop a KLA-based cancer therapy, KLA-iRGD, a recombinant protein was constructed. It consists of the KLA peptide and iRGD (CRGDKGPDC), a tumor-homing peptide with high penetration into tumor tissue and cells. The conjugated KLA exhibits pro-apoptotic activity to prevent the growth of a tumor once it is inside the cell. Once KLA-iRGD is internalized in cultured tumor cells, via the activation of the receptor neuropilin-1, it spreads extensively throughout the mass of the tumor. The recombinant KLA-iRGD protein showed antitumor activity in vivo in mice and in vitro in tumor cell lines. Repeated treatment with KLA-iRGD greatly prevented tumor growth, resulting in a considerable reduction in tumor volume. According to our data, KLA-iRGD may serve as a potential anticancer agent with limited systemic toxicity and high selectivity for the treatment of MKN45 gastric cancer, which may lead to the enhancement of new targeted anticancer agents.
View Figures
View References

Related Articles

Journal Cover

April-2017
Volume 37 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Li X, Sha H, Zhang L, Bian X, Han X and Liu B: Tumor-penetrating peptide fused to a pro-apoptotic peptide facilitates effective gastric cancer therapy. Oncol Rep 37: 2063-2070, 2017
APA
Huang, Y., Li, X., Sha, H., Zhang, L., Bian, X., Han, X., & Liu, B. (2017). Tumor-penetrating peptide fused to a pro-apoptotic peptide facilitates effective gastric cancer therapy. Oncology Reports, 37, 2063-2070. https://doi.org/10.3892/or.2017.5440
MLA
Huang, Y., Li, X., Sha, H., Zhang, L., Bian, X., Han, X., Liu, B."Tumor-penetrating peptide fused to a pro-apoptotic peptide facilitates effective gastric cancer therapy". Oncology Reports 37.4 (2017): 2063-2070.
Chicago
Huang, Y., Li, X., Sha, H., Zhang, L., Bian, X., Han, X., Liu, B."Tumor-penetrating peptide fused to a pro-apoptotic peptide facilitates effective gastric cancer therapy". Oncology Reports 37, no. 4 (2017): 2063-2070. https://doi.org/10.3892/or.2017.5440