Open Access

miR-539 inhibits FSCN1 expression and suppresses hepatocellular carcinoma migration and invasion

  • Authors:
    • Yanping Liu
    • Wei Hong
    • Cancan Zhou
    • Zhengdong Jiang
    • Guanghui Wang
    • Guangbing Wei
    • Xuqi Li
  • View Affiliations

  • Published online on: April 3, 2017     https://doi.org/10.3892/or.2017.5549
  • Pages: 2593-2602
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Increasing evidence indicates that the dysregulation of miRNAs that act as tumor suppressors or oncogenes is involved in tumorigenesis. However, the role of miR-539 in hepatocellular carcinoma (HCC) has not been well investigated. Quantitative RT-PCR (qRT-PCR), proliferation assay, colony formation assay, migration and invasion assays, western blotting, and xenograft tumor growth models were performed to assess the expression levels and functions of miR-539 in HCC. Luciferase reporter assays, qRT-PCR, western blotting, and immunohistochemistry were used to identify and verify the targets of miR-539. miR-539 was significantly downregulated in HCC cell lines and tissue samples. Ectopic expression of miR-539 inhibited cell viability, proliferation, migration, and invasion in vitro and suppressed xenograft tumor growth in vivo. Fascin homologue 1 (FSCN1) was verified as a direct target of miR-539, and overexpression of FSCN1 promoted HCC cell migration and invasion. miR-539 acts as a novel tumor suppressor in the development and progression of HCC by targeting FSCN1, providing new insight into the mechanisms of HCC carcinogenesis and suggesting that miR-539 may be a therapeutic target.
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May-2017
Volume 37 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Copy and paste a formatted citation
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Spandidos Publications style
Liu Y, Hong W, Zhou C, Jiang Z, Wang G, Wei G and Li X: miR-539 inhibits FSCN1 expression and suppresses hepatocellular carcinoma migration and invasion. Oncol Rep 37: 2593-2602, 2017
APA
Liu, Y., Hong, W., Zhou, C., Jiang, Z., Wang, G., Wei, G., & Li, X. (2017). miR-539 inhibits FSCN1 expression and suppresses hepatocellular carcinoma migration and invasion. Oncology Reports, 37, 2593-2602. https://doi.org/10.3892/or.2017.5549
MLA
Liu, Y., Hong, W., Zhou, C., Jiang, Z., Wang, G., Wei, G., Li, X."miR-539 inhibits FSCN1 expression and suppresses hepatocellular carcinoma migration and invasion". Oncology Reports 37.5 (2017): 2593-2602.
Chicago
Liu, Y., Hong, W., Zhou, C., Jiang, Z., Wang, G., Wei, G., Li, X."miR-539 inhibits FSCN1 expression and suppresses hepatocellular carcinoma migration and invasion". Oncology Reports 37, no. 5 (2017): 2593-2602. https://doi.org/10.3892/or.2017.5549