miR-1247-5p functions as a tumor suppressor in human hepatocellular carcinoma by targeting Wnt3

  • Authors:
    • Yuankui Chu
    • Weining Fan
    • Wenwei Guo
    • Yixin Zhang
    • Lixin Wang
    • Le Guo
    • Xiangguo Duan
    • Jun Wei
    • Guangxian Xu
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  • Published online on: June 6, 2017     https://doi.org/10.3892/or.2017.5702
  • Pages: 343-351
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Abstract

Increasing evidence suggests that aberrant expression of certain microRNAs (miRNAs) may participate in the genesis and progression of tumors. Several studies have indicated that miR-1247-5p plays different roles in various types of cancer cells. The effects of miR-1247-5p on human hepatocellular carcinoma (HCC) cells are elusive. In the present study, we investigated the effects of miR-1247-5p on the progression of HCC. The transcript of miR-1247-5p was markedly downregulated in clinical samples of patients with HCC and HCC cell lines, and ectopic overexpression of miR‑1247-5p markedly inhibited the proliferation and invasion of HepG2 cells, induced cell apoptosis in vitro, and suppressed the growth of transplanted tumors in vivo. Wnt3 was found to be a potential target of miR-1247-5p and overexpression of miR-1247-5p was able to significantly downregulate the expression of Wnt3 by directly targeting the 3'UTR of this gene, which was verified by luciferase reporter assay and western blotting. Furthermore, we found that the miR-1247-5p gene was hypermethylated in HepG2 cells, and the transcript of miR-1247-5p was increased significantly after treatment with the demethylation drug 5-azacytidine. These findings demonstrated that miR-1247-5p functions as a tumor suppressor in human HCC by targeting Wnt3 and that the expression of miR-1247-5p can be regulated by DNA methylation, which indicates that miR-1247-5p has the potential to be a therapeutic target as well as a diagnostic marker of HCC.
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July-2017
Volume 38 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chu Y, Fan W, Guo W, Zhang Y, Wang L, Guo L, Duan X, Wei J and Xu G: miR-1247-5p functions as a tumor suppressor in human hepatocellular carcinoma by targeting Wnt3. Oncol Rep 38: 343-351, 2017
APA
Chu, Y., Fan, W., Guo, W., Zhang, Y., Wang, L., Guo, L. ... Xu, G. (2017). miR-1247-5p functions as a tumor suppressor in human hepatocellular carcinoma by targeting Wnt3. Oncology Reports, 38, 343-351. https://doi.org/10.3892/or.2017.5702
MLA
Chu, Y., Fan, W., Guo, W., Zhang, Y., Wang, L., Guo, L., Duan, X., Wei, J., Xu, G."miR-1247-5p functions as a tumor suppressor in human hepatocellular carcinoma by targeting Wnt3". Oncology Reports 38.1 (2017): 343-351.
Chicago
Chu, Y., Fan, W., Guo, W., Zhang, Y., Wang, L., Guo, L., Duan, X., Wei, J., Xu, G."miR-1247-5p functions as a tumor suppressor in human hepatocellular carcinoma by targeting Wnt3". Oncology Reports 38, no. 1 (2017): 343-351. https://doi.org/10.3892/or.2017.5702