Coptidis Rhizoma induces intrinsic apoptosis through BAX and BAK activation in human melanoma

  • Authors:
    • Xiaoou Xu
    • Satoru Yokoyama
    • Yoshihiro Hayakawa
    • Ikuo Saiki
  • View Affiliations

  • Published online on: May 26, 2017     https://doi.org/10.3892/or.2017.5672
  • Pages: 538-544
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Abstract

Malignant melanoma has exhibited a rising incidence in recent years worldwide. Although various molecular targeted drugs are being researched and developed for melanoma patients, their efficacy appears to be unsatisfactory. Over the past few years, several reports have demonstrated that Coptidis Rhizoma water extracts (CR) or its major active chemical component, berberine, has anticancer activities in various types of cancer, including melanoma. However, their underlying mechanisms have not been well understood. In the present study, we determined that CR suppressed melanoma cell viability, which was mainly mediated through apoptosis. In addition, the expression levels of anti-apoptotic proteins, BCL2A1, MCL1 and BCL-w, were strongly suppressed by CR treatment. Furthermore, multi-domain pro-apoptotic proteins BAX and BAK were activated by CR treatment and were also required for the CR-induced apoptosis. Collectively, CR or some formulations containing CR, may be effective safe treatment strategies for human melanoma.
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July-2017
Volume 38 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Xu X, Yokoyama S, Hayakawa Y and Saiki I: Coptidis Rhizoma induces intrinsic apoptosis through BAX and BAK activation in human melanoma. Oncol Rep 38: 538-544, 2017
APA
Xu, X., Yokoyama, S., Hayakawa, Y., & Saiki, I. (2017). Coptidis Rhizoma induces intrinsic apoptosis through BAX and BAK activation in human melanoma. Oncology Reports, 38, 538-544. https://doi.org/10.3892/or.2017.5672
MLA
Xu, X., Yokoyama, S., Hayakawa, Y., Saiki, I."Coptidis Rhizoma induces intrinsic apoptosis through BAX and BAK activation in human melanoma". Oncology Reports 38.1 (2017): 538-544.
Chicago
Xu, X., Yokoyama, S., Hayakawa, Y., Saiki, I."Coptidis Rhizoma induces intrinsic apoptosis through BAX and BAK activation in human melanoma". Oncology Reports 38, no. 1 (2017): 538-544. https://doi.org/10.3892/or.2017.5672