Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K‑Ras‑p110α interaction

  • Authors:
    • Min Gao
    • Kai-Min Guo
    • Ying-Mei Wei
    • Ming-Ming Ma
    • Jia-Rong Cai
    • Ting-Ting Xia
    • Qing-Jian Ye
  • View Affiliations

  • Published online on: August 22, 2017     https://doi.org/10.3892/or.2017.5915
  • Pages: 2507-2517
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Abstract

Aspirin has been confirmed as an effective antitumor drug in various cancers. However, the relationship between aspirin and uterine leiomyoma is still underexplored. Here, we explored the effects of aspirin on human uterine leiomyoma cells and provide insights into the underlying mechanisms. Cell Counting Kit-8 (CCK-8) and flow cytometry analysis showed that aspirin treatment inhibited cell proliferation and promoted cell cycle arrest at G0/G1 phase in a dose- and time‑dependent manner of human uterine leiomyoma cells. Further studies revealed that aspirin blocked the interaction between K-Ras and p110α by co-immunoprecipitation and immunofluorescence. Western blotting demonstrated K‑Ras‑p110α interaction was required for the effects of aspirin‑induced inhibition on cell growth and cell cycle transition via cell cycle regulators, including cyclin D1 and cyclin-dependent kinase 2 (CDK2). PI3K/Akt/caspase signaling pathway was involved in human uterine leiomyoma cell growth under aspirin treatment. Taken together, these results suggest that aspirin inhibited human uterine leiomyoma cell growth by regulating K‑Ras‑p110α interaction. Aspirin which targeting on interaction between K-Ras and p110α may serve as a new therapeutic drug for uterine leiomyoma treatment.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Gao M, Guo K, Wei Y, Ma M, Cai J, Xia T and Ye Q: Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K‑Ras‑p110α interaction. Oncol Rep 38: 2507-2517, 2017
APA
Gao, M., Guo, K., Wei, Y., Ma, M., Cai, J., Xia, T., & Ye, Q. (2017). Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K‑Ras‑p110α interaction. Oncology Reports, 38, 2507-2517. https://doi.org/10.3892/or.2017.5915
MLA
Gao, M., Guo, K., Wei, Y., Ma, M., Cai, J., Xia, T., Ye, Q."Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K‑Ras‑p110α interaction". Oncology Reports 38.4 (2017): 2507-2517.
Chicago
Gao, M., Guo, K., Wei, Y., Ma, M., Cai, J., Xia, T., Ye, Q."Aspirin inhibits the proliferation of human uterine leiomyoma cells by downregulation of K‑Ras‑p110α interaction". Oncology Reports 38, no. 4 (2017): 2507-2517. https://doi.org/10.3892/or.2017.5915