Wild-type p53 controls the level of fibronectin expression in breast cancer cells

  • Authors:
    • Daeun You
    • Seung Pil Jung
    • Yisun Jeong
    • Soo Youn Bae
    • Sangmin Kim
  • View Affiliations

  • Published online on: July 31, 2017     https://doi.org/10.3892/or.2017.5860
  • Pages: 2551-2557
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Abstract

Aberrant fibronectin (FN) expression is associated with poor prognosis, cell adhesion, and cell motility in a variety of cancer cells. In this study, we investigated the relationship between p53 and FN expression in breast cancer cells. Basal FN expression was significantly decreased by treatment with the p53 activator III, RITA, in MCF7 breast cancer cells with wild-type p53. In addition, overexpression of wild-type p53 markedly decreased the level of FN expression in p53-mutant breast cancer cells. To examine the mechanism underlying the relationship between p53 and FN expression, we treated MCF7 breast cancer cells with the tumor promoter TPA (12-O-tetradecanoylphorbol-13-acetate). Our results showed that basal FN expression was increased by TPA treatment in a time-dependent manner. In contrast, the level of p53 expression was decreased by TPA treatment. However, the expression of FN and p53 was not altered by TPA in p53-mutant breast cancer cells. Furthermore, the alterations in FN and p53 expression in response to TPA were prevented by a specific MEK inhibitor, UO126. Finally, we demonstrated that TPA triggers degradation of p53 through the proteasomal pathway in MCF7 cells. TPA-induced FN expression was decreased by the proteasome inhibitor MG132. Under the same condition, p53 protein expression, but not mRNA expression, was reversed by MG132. Taken together, our data demonstrate that the level of FN expression is associated with the status and expression of p53 in breast cancer cells.
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October-2017
Volume 38 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
You D, Jung SP, Jeong Y, Bae SY and Kim S: Wild-type p53 controls the level of fibronectin expression in breast cancer cells. Oncol Rep 38: 2551-2557, 2017
APA
You, D., Jung, S.P., Jeong, Y., Bae, S.Y., & Kim, S. (2017). Wild-type p53 controls the level of fibronectin expression in breast cancer cells. Oncology Reports, 38, 2551-2557. https://doi.org/10.3892/or.2017.5860
MLA
You, D., Jung, S. P., Jeong, Y., Bae, S. Y., Kim, S."Wild-type p53 controls the level of fibronectin expression in breast cancer cells". Oncology Reports 38.4 (2017): 2551-2557.
Chicago
You, D., Jung, S. P., Jeong, Y., Bae, S. Y., Kim, S."Wild-type p53 controls the level of fibronectin expression in breast cancer cells". Oncology Reports 38, no. 4 (2017): 2551-2557. https://doi.org/10.3892/or.2017.5860