Open Access

Inhibitor of β-catenin and TCF (ICAT) promotes cervical cancer growth and metastasis by disrupting E-cadherin/β-catenin complex

  • Authors:
    • Yayun Jiang
    • Wei Ren
    • Weijia Wang
    • Jing Xia
    • Liyao Gou
    • Mengyao Liu
    • Qun Wan
    • Lan Zhou
    • Yaguang Weng
    • Tongchuan He
    • Yan Zhang
  • View Affiliations

  • Published online on: September 18, 2017     https://doi.org/10.3892/or.2017.5962
  • Pages: 2597-2606
  • Copyright: © Jiang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The inhibitor of β-catenin and TCF (ICAT) blocks the binding of TCF to β-catenin and has been demonstrated as a suppressor of the Wnt/β-catenin signaling pathway. It has been reported to exert a different function around a wide variety of cancers. However, its function and underlying mechanisms in human cervical cancer remains unknown. In the present study, the expression of ICAT in 41 human cervical cancer tissues and 30 normal cervical tissues was evaluated by immunohistochemical analysis. ICAT was found highly expressed in cancer tissues. ICAT overexpression significantly promoted SiHa cell proliferation in vitro by causing G1 arrest, and enhanced cell migration and invasion whereas, ICAT knockdown induced opposite effects in Caski cells which have higher expression of ICAT. Downregulation or overexpression of ICAT resulted in an altered expression of the epithelial-mesenchymal transition (EMT). Furthermore, immunoprecipitation assays revealed that ICAT pormoted cervical cancer EMT by competing in E-cadhenin binding to β-caterin. Overexpression of ICAT in SiHa cells promoted tumor growth and EMT was also demonstrated by the xenograft mouse experiment. These results demonstrate that ICAT contributed to the progression of cervical cancer and may play a role in the regulation of EMT by distrupting the E-cadherin/β-catenin complex. It may be a novel potential therapeutic target for therapy in human cervical cancer.
View Figures
View References

Related Articles

Journal Cover

November-2017
Volume 38 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Jiang Y, Ren W, Wang W, Xia J, Gou L, Liu M, Wan Q, Zhou L, Weng Y, He T, He T, et al: Inhibitor of β-catenin and TCF (ICAT) promotes cervical cancer growth and metastasis by disrupting E-cadherin/β-catenin complex. Oncol Rep 38: 2597-2606, 2017
APA
Jiang, Y., Ren, W., Wang, W., Xia, J., Gou, L., Liu, M. ... Zhang, Y. (2017). Inhibitor of β-catenin and TCF (ICAT) promotes cervical cancer growth and metastasis by disrupting E-cadherin/β-catenin complex. Oncology Reports, 38, 2597-2606. https://doi.org/10.3892/or.2017.5962
MLA
Jiang, Y., Ren, W., Wang, W., Xia, J., Gou, L., Liu, M., Wan, Q., Zhou, L., Weng, Y., He, T., Zhang, Y."Inhibitor of β-catenin and TCF (ICAT) promotes cervical cancer growth and metastasis by disrupting E-cadherin/β-catenin complex". Oncology Reports 38.5 (2017): 2597-2606.
Chicago
Jiang, Y., Ren, W., Wang, W., Xia, J., Gou, L., Liu, M., Wan, Q., Zhou, L., Weng, Y., He, T., Zhang, Y."Inhibitor of β-catenin and TCF (ICAT) promotes cervical cancer growth and metastasis by disrupting E-cadherin/β-catenin complex". Oncology Reports 38, no. 5 (2017): 2597-2606. https://doi.org/10.3892/or.2017.5962