Low heme oxygenase-1 expression promotes gastric cancer cell apoptosis, inhibits proliferation and invasion, and correlates with increased overall survival in gastric cancer patients

  • Authors:
    • Quan-Guang Ren
    • Sheng-Li Yang
    • Pin-Dong Li
    • Jin-Rong Xiong
    • Xiefan Fang
    • Jian-Li Hu
    • Qiu-Shuang Wang
    • Ren-Wang Chen
    • Ye-Shan Chen
    • Lu Wen
    • Miao Peng
  • View Affiliations

  • Published online on: September 19, 2017     https://doi.org/10.3892/or.2017.5967
  • Pages: 2852-2858
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Abstract

Heme oxygenase-1 (HO-1) plays a key role in anti-oxidation, anti-apoptosis, and anti-proliferation in various types of cancers. However, the relationship between HO-1 expression and gastric cancer development remains largely unknown. In this study, the protein expression of HO-1 in human gastric cancer was measured by immunohistochemistry on paraffin sections of 89 paired gastric carcinoma tissues and adjacent non-cancer tissues. The correlation of HO-1 expression with 5-year overall survival rate was estimated. The effects of decreased HO-1 expression by two strands of small interfered RNAs (siRNAs) on cell apoptosis, proliferation, and invasion of gastric cancer cell lines were examined by flow cytometry, the MTT assay, and the cell migration assay, respectively. High expression of HO-1 was detected in 11.2% (10/89) of gastric carcinoma tissues, compared with 1.1% (1/89) in matched adjacent normal tissues, and correlated with a decreased survival rate in gastric cancer patients. There were no significant correlations between HO-1 expression and clinical characteristics. Downregulation of HO-1 expression using two strands of siRNAs promoted apoptosis and inhibited the proliferation and invasion of two gastric cancer cell lines, SGC7901 and MKN-28 cells. This study demonstrated that HO-1 plays a vital role in the development of gastric cancer and may serve as a therapeutic target of this type of cancer.
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November-2017
Volume 38 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ren Q, Yang S, Li P, Xiong J, Fang X, Hu J, Wang Q, Chen R, Chen Y, Wen L, Wen L, et al: Low heme oxygenase-1 expression promotes gastric cancer cell apoptosis, inhibits proliferation and invasion, and correlates with increased overall survival in gastric cancer patients. Oncol Rep 38: 2852-2858, 2017
APA
Ren, Q., Yang, S., Li, P., Xiong, J., Fang, X., Hu, J. ... Peng, M. (2017). Low heme oxygenase-1 expression promotes gastric cancer cell apoptosis, inhibits proliferation and invasion, and correlates with increased overall survival in gastric cancer patients. Oncology Reports, 38, 2852-2858. https://doi.org/10.3892/or.2017.5967
MLA
Ren, Q., Yang, S., Li, P., Xiong, J., Fang, X., Hu, J., Wang, Q., Chen, R., Chen, Y., Wen, L., Peng, M."Low heme oxygenase-1 expression promotes gastric cancer cell apoptosis, inhibits proliferation and invasion, and correlates with increased overall survival in gastric cancer patients". Oncology Reports 38.5 (2017): 2852-2858.
Chicago
Ren, Q., Yang, S., Li, P., Xiong, J., Fang, X., Hu, J., Wang, Q., Chen, R., Chen, Y., Wen, L., Peng, M."Low heme oxygenase-1 expression promotes gastric cancer cell apoptosis, inhibits proliferation and invasion, and correlates with increased overall survival in gastric cancer patients". Oncology Reports 38, no. 5 (2017): 2852-2858. https://doi.org/10.3892/or.2017.5967