Open Access

Knockdown of lncRNA-XIST enhances the chemosensitivity of NSCLC cells via suppression of autophagy

  • Authors:
    • Wei Sun
    • Yukun Zu
    • Xiangning Fu
    • Yu Deng
  • View Affiliations

  • Published online on: October 24, 2017     https://doi.org/10.3892/or.2017.6056
  • Pages:3347-3354
  • Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Drug resistance is the major factor contributing to the failure of chemotherapy in non-small cell lung cancer (NSCLC) patients. Emerging evidence suggests that autophagy plays a vital role in the chemoresistance of many types of tumors. However, the exact mechanism underlying the chemoresistance of NSCLC is still elusive, and it is unclear whether lncRNA-XIST is involved in autophagy and chemoresistance of NSCLC. In the present study, we demonstrated that lncRNA-XIST was overexpressed in NSCLC tumor samples, and knockdown of lncRNA-XIST significantly decreased autophagy by regulation of ATG7 as determined by qPCR and by western blotting. Furthermore, we found that miR-17 was upregulated following knockdown of lncRNA-XIST, and miR-17 mimics decreased the protein levels of ATG7 by directly targeting the 3'-untranslated region of ATG7 mRNA as determined by RT-qPCR and by western blotting. Furthermore, we found that the expression level of lncRNA-XIST was markedly increased in cisplatin-resistant A549 cells as determined by q-PCR. Knockdown of lncRNA-XIST restored the chemosensitivity of cisplatin-resistant A549 cells to cisplatin, which was reversed by miR-17 inhibitor and overexpression of ATG7 as determined by CCK8 assays. This study provides evidence that lncRNA-XIST may be a potential marker of poor response to cisplatin chemotherapy in NSCLC patients and the pathway ‘lncRNA-XIST/miR-17/autophagy’ may be a promising target for patients with chemoresistant NSCLC.

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Journal Cover

December 2017
Volume 38 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
Ranked #31/217 Oncology
(total number of cites)

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APA
Sun, W., Zu, Y., Fu, X., & Deng, Y. (2017). Knockdown of lncRNA-XIST enhances the chemosensitivity of NSCLC cells via suppression of autophagy. Oncology Reports, 38, 3347-3354. https://doi.org/10.3892/or.2017.6056
MLA
Sun, W., Zu, Y., Fu, X., Deng, Y."Knockdown of lncRNA-XIST enhances the chemosensitivity of NSCLC cells via suppression of autophagy". Oncology Reports 38.6 (2017): 3347-3354.
Chicago
Sun, W., Zu, Y., Fu, X., Deng, Y."Knockdown of lncRNA-XIST enhances the chemosensitivity of NSCLC cells via suppression of autophagy". Oncology Reports 38, no. 6 (2017): 3347-3354. https://doi.org/10.3892/or.2017.6056