Open Access

PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells

  • Authors:
    • Weidong Liu
    • Xuxu Liu
    • Lei Wang
    • Bin Zhu
    • Chang Zhang
    • Wei Jia
    • Hecheng Zhu
    • Xingdong Liu
    • Meizuo Zhong
    • Dan Xie
    • Yanyu Liu
    • Shasha Li
    • Jia Shi
    • Jianxing Lin
    • Xiaomeng Xia
    • Xingjun Jiang
    • Caiping Ren
  • View Affiliations

  • Published online on: November 6, 2017     https://doi.org/10.3892/or.2017.6080
  • Pages:45-52
  • Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Phospholipase C (PLC) is a pivotal enzyme in the phosphoinositide pathway that promotes the second messengers, diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3), to participate in eukaryotic signal transduction. Several PLC isozymes are associated with cancer, such as PLC-β1, PLC-δ1, PLC-ε and PLC-γ1. However, the role of PLC-δ3 (PLCD3) in nasopharyngeal carcinoma (NPC) has not been investigated to date. In our previous study, we demonstrated that flotillin2 (Flot2) plays a pro-neoplastic role in NPC and is involved in tumour progression and metastasis. In the present study, we screened the interacting proteins of Flot2 using the yeast two-hybrid (Y2H) method and verified the interaction between PLCD3 and Flot2 by co-immunoprecipitation. We also investigated the biological functions of PLCD3 in NPC. Inhibition of PLCD3 expression impaired the malignant potential of 5-8F, a highly metastatic NPC cell line, by restraining its growth, proliferation, mobility and migration. The present study demonstrated that PLCD3 may be an oncogenic protein in NPC and that it plays an important role in the progression of NPC partially by interacting with Flot2.

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January 2018
Volume 39 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

2016 Impact Factor: 2.662
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APA
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W. ... Ren, C. (2018). PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells. Oncology Reports, 39, 45-52. https://doi.org/10.3892/or.2017.6080
MLA
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W., Zhu, H., Liu, X., Zhong, M., Xie, D., Liu, Y., Li, S., Shi, J., Lin, J., Xia, X., Jiang, X., Ren, C."PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells". Oncology Reports 39.1 (2018): 45-52.
Chicago
Liu, W., Liu, X., Wang, L., Zhu, B., Zhang, C., Jia, W., Zhu, H., Liu, X., Zhong, M., Xie, D., Liu, Y., Li, S., Shi, J., Lin, J., Xia, X., Jiang, X., Ren, C."PLCD3, a flotillin2-interacting protein, is involved in proliferation, migration and invasion of nasopharyngeal carcinoma cells". Oncology Reports 39, no. 1 (2018): 45-52. https://doi.org/10.3892/or.2017.6080