Enhanced production of reactive oxygen species in HeLa cells under concurrent low‑dose carboplatin and Photofrin® photodynamic therapy

  • Authors:
    • Younshick Choi
    • Ji‑Eun Chang
    • Sanghoon Jheon
    • Sei‑Jun Han
    • Jong‑Ki Kim
  • View Affiliations

  • Published online on: May 3, 2018     https://doi.org/10.3892/or.2018.6415
  • Pages: 339-345
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Abstract

Concurrent low‑dose carboplatin/Photofrin® photodynamic therapy (ccPDT) has been shown to promote relapse‑free complete tumor regression in cervical or endometrial cancer patients as a fertility‑preservation therapy. This study aimed to investigate the molecular mechanism of the enhanced therapeutic efficacy of ccPDT by determining intracellular reactive oxygen species (ROS) and necrotic or apoptotic cell damage in HeLa cells loaded with fluorescent oxidant agents and Photofrin or/and carboplatin under light irradiation. The cytotoxic effects of ccPDT were compared when monitored with a light dose under carboplatin or Photofrin alone. Photofrin‑PDT alone did not enhance either hydroxyl radicals (OH•) or superoxide anions (O2•–), but a slight enhancement of hydrogen peroxide (H2O2) production was observed. A larger enhancement of ROS production was obtained in a dose‑dependent manner following ccPDT, especially OH• and H2O2, in conjunction with both necrotic and apoptotic cell death, compared with necrotic‑prone PDT alone. The carboplatin‑mediated Fenton reaction: 2[PtII]2 + H2O2 → [Pt2.25]4 + OH¯+ OH• was proposed to explain the dose‑dependent enhancement of OH•. In conclusion, the therapeutic enhancement of ccPDT in vitro was attributable to the carboplatin‑mediated synergetic production of OH▪ and apoptotic cellular damage, compared with Photofrin‑PDT alone.
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July-2018
Volume 40 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Choi Y, Chang JE, Jheon S, Han SJ and Kim JK: Enhanced production of reactive oxygen species in HeLa cells under concurrent low‑dose carboplatin and Photofrin® photodynamic therapy. Oncol Rep 40: 339-345, 2018
APA
Choi, Y., Chang, J., Jheon, S., Han, S., & Kim, J. (2018). Enhanced production of reactive oxygen species in HeLa cells under concurrent low‑dose carboplatin and Photofrin® photodynamic therapy. Oncology Reports, 40, 339-345. https://doi.org/10.3892/or.2018.6415
MLA
Choi, Y., Chang, J., Jheon, S., Han, S., Kim, J."Enhanced production of reactive oxygen species in HeLa cells under concurrent low‑dose carboplatin and Photofrin® photodynamic therapy". Oncology Reports 40.1 (2018): 339-345.
Chicago
Choi, Y., Chang, J., Jheon, S., Han, S., Kim, J."Enhanced production of reactive oxygen species in HeLa cells under concurrent low‑dose carboplatin and Photofrin® photodynamic therapy". Oncology Reports 40, no. 1 (2018): 339-345. https://doi.org/10.3892/or.2018.6415