Transcutaneous carbon dioxide enhances the antitumor effect of radiotherapy on oral squamous cell carcinoma

  • Authors:
    • Eiji Iwata
    • Takumi Hasegawa
    • Takeshi Ueha
    • Daisuke Takeda
    • Izumi Saito
    • Teruya Kawamoto
    • Toshihiro Akisue
    • Yoshitada Sakai
    • Ryohei Sasaki
    • Ryosuke Kuroda
    • Takahide Komori
  • View Affiliations

  • Published online on: May 16, 2018     https://doi.org/10.3892/or.2018.6444
  • Pages: 434-442
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Abstract

Radiotherapy (RT) is one of the main treatment modalities for oral squamous cell carcinoma (OSCC), however, radioresistance is a major impediment to its clinical success and poses as a concern that needs to be addressed. Tumor hypoxia is known to be significantly associated with radioresistance in various malignancies, hence, resolving the hypoxic state of a tumor may improve the antitumor effect of RT on OSCC. We have previously revealed that transcutaneous CO2 induced mitochondrial apoptosis and suppressed tumor growth in OSCC by resolving hypoxia. Considering the previous study, we hypothesized that transcutaneous CO2 may enhance the antitumor effect of RT on OSCC by improving intratumoral hypoxia, thereby overcoming radioresistance. In the present study, the combination of transcutaneous CO2 and RT significantly inhibited tumor growth compared with other treatments. This combination therapy also led to decreased expression of HIF-1α in parallel with increased expression of the cleaved forms of caspase-3-8-9 and PARP, which play essential roles in mitochondrial apoptosis. Additionally, the combination therapy increased the expression of ROS modulator 1 and subsequent mitochondrial ROS production, compared to RT alone. These results indicated that transcutaneous CO2 could potentially improve the antitumor effect of RT by decreasing the intratumoral hypoxia and increasing the mitochondrial apoptosis. Our findings indicated that CO2 therapy may be a novel adjuvant therapy in combination with RT for OSCC.
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July-2018
Volume 40 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Iwata E, Hasegawa T, Ueha T, Takeda D, Saito I, Kawamoto T, Akisue T, Sakai Y, Sasaki R, Kuroda R, Kuroda R, et al: Transcutaneous carbon dioxide enhances the antitumor effect of radiotherapy on oral squamous cell carcinoma. Oncol Rep 40: 434-442, 2018
APA
Iwata, E., Hasegawa, T., Ueha, T., Takeda, D., Saito, I., Kawamoto, T. ... Komori, T. (2018). Transcutaneous carbon dioxide enhances the antitumor effect of radiotherapy on oral squamous cell carcinoma. Oncology Reports, 40, 434-442. https://doi.org/10.3892/or.2018.6444
MLA
Iwata, E., Hasegawa, T., Ueha, T., Takeda, D., Saito, I., Kawamoto, T., Akisue, T., Sakai, Y., Sasaki, R., Kuroda, R., Komori, T."Transcutaneous carbon dioxide enhances the antitumor effect of radiotherapy on oral squamous cell carcinoma". Oncology Reports 40.1 (2018): 434-442.
Chicago
Iwata, E., Hasegawa, T., Ueha, T., Takeda, D., Saito, I., Kawamoto, T., Akisue, T., Sakai, Y., Sasaki, R., Kuroda, R., Komori, T."Transcutaneous carbon dioxide enhances the antitumor effect of radiotherapy on oral squamous cell carcinoma". Oncology Reports 40, no. 1 (2018): 434-442. https://doi.org/10.3892/or.2018.6444