G‑protein‑coupled receptor 120 regulates the development and progression of human esophageal cancer

  • Authors:
    • Zhen Cui
    • Duojie Li
    • Jingjing Liu
    • Yajun Zhang
    • Hongbo Xu
    • Hongmei Yin
    • Hongwei Li
    • Gengming Wang
    • Hanfei Cai
    • Lei Zhang
    • Shimiao Duan
    • Hao Jiang
  • View Affiliations

  • Published online on: May 31, 2018     https://doi.org/10.3892/or.2018.6470
  • Pages: 1147-1155
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Abstract

The aim of the present study was to investigate the role of G‑protein coupled receptor 120 (GPR120) in esophageal cancer and explore the related mechanisms. The expression of GPR120 in esophageal cancer tissues was examined by immunohistochemistry. Correlation analysis was performed to investigate the association between the level of GPR120 and clinical parameters. The expression of GPR120 was evaluated in esophageal cancer cell lines and the effects of GPR120 on cell proliferation, clone formation, migration and invasion were evaluated in an in vitro cell model and an in vivo ectopic tumor nude mice model. In addition, the effect of GPR120 on epithelial‑mesenchymal transition (EMT), PI3K and I‑κB pathway, as well as angiogenesis and inflammation‑related cytokines was explored in order to elucidate the underlying mechanisms. Significantly increased expression of GPR120 was observed in esophageal cancer tissues compared to normal tissues. The expression of GPR120 was significantly related with histological grade, TNM stage and lymph node metastasis. GPR120 knockdown significantly decreased cell proliferation, clone formation, migration and invasion in vitro and decreased tumor growth in vivo. Furthermore significantly increased levels of E‑cadherin and decreased levels of N‑cadherin and vimentin, decreased level of Akt phosphorylation and I‑κB phosphorylation, as well as decreased levels of vascular endothelial growth factor (VEGF), interleukin‑8 (IL‑8) and cyclooxygenase‑2 (Cox‑2) and its corresponding protein PGE2 were observed as the underlying mechanisms. In conclusion, we observed an increased level of GPR120 in esophageal cancer tissues, which served as a positive regulator of the development and progression of esophageal cancer. Multiple mechanisms including EMT, PI3K and I‑κB pathway, as well as angiogenesis and inflammation‑related cytokines were involved.
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August-2018
Volume 40 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Cui Z, Li D, Liu J, Zhang Y, Xu H, Yin H, Li H, Wang G, Cai H, Zhang L, Zhang L, et al: G‑protein‑coupled receptor 120 regulates the development and progression of human esophageal cancer. Oncol Rep 40: 1147-1155, 2018
APA
Cui, Z., Li, D., Liu, J., Zhang, Y., Xu, H., Yin, H. ... Jiang, H. (2018). G‑protein‑coupled receptor 120 regulates the development and progression of human esophageal cancer. Oncology Reports, 40, 1147-1155. https://doi.org/10.3892/or.2018.6470
MLA
Cui, Z., Li, D., Liu, J., Zhang, Y., Xu, H., Yin, H., Li, H., Wang, G., Cai, H., Zhang, L., Duan, S., Jiang, H."G‑protein‑coupled receptor 120 regulates the development and progression of human esophageal cancer". Oncology Reports 40.2 (2018): 1147-1155.
Chicago
Cui, Z., Li, D., Liu, J., Zhang, Y., Xu, H., Yin, H., Li, H., Wang, G., Cai, H., Zhang, L., Duan, S., Jiang, H."G‑protein‑coupled receptor 120 regulates the development and progression of human esophageal cancer". Oncology Reports 40, no. 2 (2018): 1147-1155. https://doi.org/10.3892/or.2018.6470