Open Access

Interaction of hematopoietic CD34+ CD45+ stem cells and cancer cells stimulated by TGF‑β1 in a model of glioblastoma in vitro

  • Authors:
    • Elena Milkina
    • Arina Ponomarenko
    • Maria Korneyko
    • Irina Lyakhova
    • Yulia Zayats
    • Sergey Zaitsev
    • Polina Mischenko
    • Marina Eliseikina
    • Yuri Khotimchenko
    • Valeryi Shevchenko
    • Hari Sharma
    • Igor Bryukhovetskiy
  • View Affiliations

  • Published online on: August 24, 2018     https://doi.org/10.3892/or.2018.6671
  • Pages: 2595-2607
  • Copyright: © Milkina et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The majority of modern treatment methods for malignant brain tumors are not sufficiently effective, with a median survival time varying between 9 and 14 months. Metastatic and invasive processes are the principal characteristics of malignant tumors. The most important pathogenic mechanism is epithelial‑mesenchymal transition (EMT), which causes epithelial cells to become more mobile, and capable of invading the surrounding tissues and migrating to distant organs. Transforming growth factor‑β1 (TGF‑β1) serves a key role in EMT‑inducing mechanisms. The current study presented the interaction between hematopoietic stem cells and glioblastoma cells stimulated by TGF‑β1 in vitro. The materials for the study were hematopoietic progenitor cell antigen CD34+ hematopoietic stem cells (HSCs) and U87 glioblastoma cells. Cell culture methods, automated monitoring of cell‑cell interactions, confocal laser microscopy, flow cytometry and electron microscopy were used. It was demonstrated that U87 cells have a complex communication system, including adhesive intercellular contacts, areas of interdigitation with dissolution of the cytoplasm, cell fusion, communication microtubes and microvesicles. TGF‑β1 affected glioblastoma cells by modifying the cell shape and intensifying their exocrine function. HSCs migrated to glioblastoma cells, interacted with them and exchanged fluorescent tags. Stimulation of cancer cells with TGF‑β1 weakened the ability of glioblastoma cells to attract HSCs and exchange a fluorescent tag. This process stimulated cancer cell proliferation, which is an indication of the ability of HSCs to ‘switch’ the proliferation and invasion processes in glioblastoma cells.
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November-2018
Volume 40 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Milkina E, Ponomarenko A, Korneyko M, Lyakhova I, Zayats Y, Zaitsev S, Mischenko P, Eliseikina M, Khotimchenko Y, Shevchenko V, Shevchenko V, et al: Interaction of hematopoietic CD34+ CD45+ stem cells and cancer cells stimulated by TGF‑β1 in a model of glioblastoma in vitro. Oncol Rep 40: 2595-2607, 2018
APA
Milkina, E., Ponomarenko, A., Korneyko, M., Lyakhova, I., Zayats, Y., Zaitsev, S. ... Bryukhovetskiy, I. (2018). Interaction of hematopoietic CD34+ CD45+ stem cells and cancer cells stimulated by TGF‑β1 in a model of glioblastoma in vitro. Oncology Reports, 40, 2595-2607. https://doi.org/10.3892/or.2018.6671
MLA
Milkina, E., Ponomarenko, A., Korneyko, M., Lyakhova, I., Zayats, Y., Zaitsev, S., Mischenko, P., Eliseikina, M., Khotimchenko, Y., Shevchenko, V., Sharma, H., Bryukhovetskiy, I."Interaction of hematopoietic CD34+ CD45+ stem cells and cancer cells stimulated by TGF‑β1 in a model of glioblastoma in vitro". Oncology Reports 40.5 (2018): 2595-2607.
Chicago
Milkina, E., Ponomarenko, A., Korneyko, M., Lyakhova, I., Zayats, Y., Zaitsev, S., Mischenko, P., Eliseikina, M., Khotimchenko, Y., Shevchenko, V., Sharma, H., Bryukhovetskiy, I."Interaction of hematopoietic CD34+ CD45+ stem cells and cancer cells stimulated by TGF‑β1 in a model of glioblastoma in vitro". Oncology Reports 40, no. 5 (2018): 2595-2607. https://doi.org/10.3892/or.2018.6671