Heterozygous ras mutations are preserved in serially passaged human tumor xenografts and established cell lines.

  • Authors:
    • K Masuda
    • H Kijima
    • H M Kim
    • S B Han
    • Y Ohnishi
    • N Sawa
    • Y Oshika
    • T Tokunaga
    • T Tsuchida
    • Y Abe
    • T Hamana
    • H Matsubayashi
    • H Yamazaki
    • N Tamaoki
    • Y Ueyama
    • M Nakamura
  • View Affiliations

  • Published online on: July 1, 1999     https://doi.org/10.3892/or.6.4.741
  • Pages: 741-746
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Abstract

We examined c-K-ras gene point mutations in human tumor xenografts and established cell lines as markers of genetic stability. Our previous study demonstrated the stability of c-K-ras gene mutations in human primary neoplasms and their tumor xenografts through serial passages in mice. In this study, we established 27 human cell lines derived from various human tumor xenografts in nude mice. Point mutation of the c-K-ras gene at codon 12 was found in 29.6% (8/27) of the cell lines, as well as in 29.6% (8/27) of the xenografts. The eight ras-mutated cell lines were derived from corresponding tumor xenografts carrying the ras mutation. Heterozygous ras gene mutation was confirmed in seven of the eight ras-mutated cell lines, as well as their corresponding xenografts. The incidence, type and heterozygosity of the c-K-ras gene mutation showed no discrepancies between the original xenografts and the established cell lines. From these findings, we concluded that point mutation of the c-K-ras gene was very stable in human tumor xenografts and established cell lines derived from the xenografts.

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Jul-Aug 1999
Volume 6 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Masuda K, Kijima H, Kim H, Han S, Ohnishi Y, Sawa N, Oshika Y, Tokunaga T, Tsuchida T, Abe Y, Abe Y, et al: Heterozygous ras mutations are preserved in serially passaged human tumor xenografts and established cell lines.. Oncol Rep 6: 741-746, 1999
APA
Masuda, K., Kijima, H., Kim, H., Han, S., Ohnishi, Y., Sawa, N. ... Nakamura, M. (1999). Heterozygous ras mutations are preserved in serially passaged human tumor xenografts and established cell lines.. Oncology Reports, 6, 741-746. https://doi.org/10.3892/or.6.4.741
MLA
Masuda, K., Kijima, H., Kim, H., Han, S., Ohnishi, Y., Sawa, N., Oshika, Y., Tokunaga, T., Tsuchida, T., Abe, Y., Hamana, T., Matsubayashi, H., Yamazaki, H., Tamaoki, N., Ueyama, Y., Nakamura, M."Heterozygous ras mutations are preserved in serially passaged human tumor xenografts and established cell lines.". Oncology Reports 6.4 (1999): 741-746.
Chicago
Masuda, K., Kijima, H., Kim, H., Han, S., Ohnishi, Y., Sawa, N., Oshika, Y., Tokunaga, T., Tsuchida, T., Abe, Y., Hamana, T., Matsubayashi, H., Yamazaki, H., Tamaoki, N., Ueyama, Y., Nakamura, M."Heterozygous ras mutations are preserved in serially passaged human tumor xenografts and established cell lines.". Oncology Reports 6, no. 4 (1999): 741-746. https://doi.org/10.3892/or.6.4.741