Silencing of the icb-1 gene inhibits the induction of differentiation-associated genes by vitamin D3 and all-trans retinoic acid in gynecological cancer cells

  • Authors:
    • Martina Haselberger
    • Anette Springwald
    • Anna Konwisorz
    • Claus Lattrich
    • Regina Goerse
    • Olaf Ortmann
    • Oliver Treeck
  • Corresponding author:
  • View Affiliations

  • Published online on: Thursday, March 31, 2011
  • Pages: 121-127 DOI: 10.3892/ijmm.2011.663

Abstract

Icb-1 (C1orf38) is a human gene initially described by our group to be involved in differentiation processes of cancer cells. To further elucidate the function of the icb-1 gene in differentiation of breast and endometrial cancer cells, we knocked down its expression by means of shRNA transfection. Knockdown of icb-1 inhibited the vitamin D3-induced up-regulation of E-cadherin expression in both MCF-7 and HEC-1B cells. Induction of E-cadherin expression by all-trans retinoic acid (ATRA) was also blocked in both cell lines expressing icb-1 siRNA. Examination of icb-1 and E-cadherin expression in 66 breast cancer tissue samples revealed a significant positive correlation between the two genes. In MCF-7 cells, silencing of the icb-1 gene inhibited the ATRA- and the vitamin D3-induced up-regulation of lactoferrin and estrogen receptor β expression. The data of our knockdown study suggest that icb-1 may act as a mediator of differentiation signals in breast cancer cells induced by ATRA or vitamin D3. These findings together with the observed co-expression of icb-1 with E-cadherin in breast cancer samples support an important role of the icb-1 gene in cancer cell differentiation.
Journal Cover

July 2011
Volume 28 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
2014 I.F. (Expected) ≥ 2.372 Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Haselberger, M., Springwald, A., Konwisorz, A., Lattrich, C., Goerse, R., Ortmann, O., & Treeck, O. (2011). Silencing of the icb-1 gene inhibits the induction of differentiation-associated genes by vitamin D3 and all-trans retinoic acid in gynecological cancer cells. International Journal of Molecular Medicine, 28, 121-127. http://dx.doi.org/10.3892/ijmm.2011.663
MLA
Haselberger, M., Springwald, A., Konwisorz, A., Lattrich, C., Goerse, R., Ortmann, O., Treeck, O."Silencing of the icb-1 gene inhibits the induction of differentiation-associated genes by vitamin D3 and all-trans retinoic acid in gynecological cancer cells". International Journal of Molecular Medicine 28.1 (2011): 121-127.
Chicago
Haselberger, M., Springwald, A., Konwisorz, A., Lattrich, C., Goerse, R., Ortmann, O., Treeck, O."Silencing of the icb-1 gene inhibits the induction of differentiation-associated genes by vitamin D3 and all-trans retinoic acid in gynecological cancer cells". International Journal of Molecular Medicine 28, no. 1 (2011): 121-127. http://dx.doi.org/10.3892/ijmm.2011.663