Reactivation of liver-specific gene expression in an immortalized human hepatocyte cell line by introduction of the human HNF4α2 gene

  • Authors:
    • Y. Inoue
    • M. Miyazaki
    • T. Tsuji
    • M. Sakaguchi
    • K. Fukaya
    • N.-H. Huh
    • M. Namba
  • View Affiliations

  • Published online on: Thursday, November 1, 2001
  • Pages: 481-487
  • DOI: 10.3892/ijmm.8.5.481

Abstract

An immortalized human hepatocyte cell line (OUMS-29) was established from fetal liver by transfection with the SV-40 large T antigen gene that has certain liver-specific functions such as albumin production and enzyme activities of CYP1A1, 1A2, and 2E1. To make OUMS-29 cells express other liver-specific functions, the human hepatocyte nuclear factor 4α2 (HNF4α2) gene was introduced into the cells, because this gene was found to be markedly down-regulated. The transduced HNF4α2 was overexpressed in the nuclei of the transfected cells, and its DNA-binding activity was also detected. The liver-specific genes such as apolipoprotein AI, CII, CIII, blood coagulation factor X, α1-antitrypsin, and HNF1α were up-regulated. Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions.
Journal Cover

November 2001
Volume 8 Issue 5

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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