Reactivation of liver-specific gene expression in an immortalized human hepatocyte cell line by introduction of the human HNF4α2 gene
- Authors: Y. Inoue, M. Miyazaki, T. Tsuji, M. Sakaguchi, K. Fukaya, N.-H. Huh, M. Namba
Published online on: Thursday, November 1, 2001
- Pages: 481-487
- DOI: 10.3892/ijmm.8.5.481
An immortalized human hepatocyte cell line (OUMS-29) was established from fetal liver by transfection with the SV-40 large T antigen gene that has certain liver-specific functions such as albumin production and enzyme activities of CYP1A1, 1A2, and 2E1. To make OUMS-29 cells express other liver-specific functions, the human hepatocyte nuclear factor 4α2 (HNF4α2) gene was introduced into the cells, because this gene was found to be markedly down-regulated. The transduced HNF4α2 was overexpressed in the nuclei of the transfected cells, and its DNA-binding activity was also detected. The liver-specific genes such as apolipoprotein AI, CII, CIII, blood coagulation factor X, α1-antitrypsin, and HNF1α were up-regulated. Thus, this cell line is expected to be a useful tool for studying the differentiated human hepatocyte functions.