Human thrombospondin 2 inhibits proliferation of microvascular endothelial cells

  • Authors: Yasushi Tomii, Junichiro Kamochi, Hitoshi Yamazaki, Nobuko Sawa, Tetsuji Tokunaga, Yasuyuki Ohnishi, Hiroshi Kijima, Yoshito Ueyama, Norikazu Tamaoki, Masato Nakamura
  • View Affiliations

  • Published online on: Friday, February 1, 2002
  • Pages: 339-342
  • DOI: 10.3892/ijo.20.2.339

Abstract

This study was performed to characterize human thrombospondin 2 (TSP2). TSP2 has recently attracted attention as an endogenous negative regulator of angiogenesis in tumorigenesis. We cloned and transfected human TSP2 cDNA into the human colon cancer cell line SW-480. Stable transfectants (TSP2-1, TSP2-6) overexpressing TSP2 were established. Growth characteristics of TSP2-transfectants were investigated in vitro and in vivo. TSP2-transfectants showed similar growth properties to vector-transfectants and wild-type SW-480 cells. The overexpression of transfected human TSP2 cDNA did not affect proliferation of SW-480 cells. When the conditioned media of TSP2-transfectants were added to cultures of bovine pulmonary microvascular endothelial cells (BPMEC), the BPMEC proliferation was significantly inhibited. These results suggested that human TSP2 is a potential inhibitor of angiogenesis.
Journal Cover

February 2002
Volume 20 Issue 2

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
(total number of cites)

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