Open Access

Tumor suppressive microRNA-375 regulates lactate dehydrogenase B in maxillary sinus squamous cell carcinoma

  • Authors:
    • Takashi Kinoshita
    • Nijiro Nohata
    • Hirofumi Yoshino
    • Toyoyuki Hanazawa
    • Naoko Kikkawa
    • Lisa Fujimura
    • Takeshi Chiyomaru
    • Kazumori Kawakami
    • Hideki Enokida
    • Masayuki Nakagawa
    • Yoshitaka Okamoto
    • Naohiko Seki
  • View Affiliations

  • Published online on: Monday, September 12, 2011
  • Pages: 185-193
  • DOI: 10.3892/ijo.2011.1196

Abstract

The expression of microRNA-375 (miR-375) is significantly reduced in cancer tissues of maxillary sinus squamous cell carcinoma (MSSCC). The aim of this study was to investigate the functional significance of miR-375 and a possible regulatory role in the MSSCC networks. Restoration of miR-375 significantly inhibited cancer cell proliferation and invasion in IMC-3 cells, suggesting that miR-375 functions as a tumor suppressor in MSSCC. Genome-wide gene expression data and luciferase reporter assays indicated that lactate dehydro­genase B (LDHB) was directly regulated by miR-375. Cancer cell proliferation and invasion were significantly inhibited by transfection of si-LDHB into IMC-3 cells, suggesting that LDHB may play a role in MSSCC oncogenic function. In clinical MSSCC specimens, LDHB mRNA levels were up-regulated in cancer tissues, which were inversely correlated with the expression of miR-375. In addition, Kaplan-Meier curves and log-rank tests revealed that the high mRNA expression levels of LDHB had a significant adverse effect on survival rate. The identification of a cancer network regulated by the miR-375 tumor suppressor could provide new insights into the molecular mechanisms of MSSCC oncogenesis.
Journal Cover

January 2012
Volume 40 Issue 1

Print ISSN: 1019-6439
Online ISSN:1791-2423

2013 Impact Factor: 2.773
Ranked #30/202 Oncology
(total number of cites)

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