Chromosome 17q25 genes, RHBDF2 and CYGB, in ovarian cancer

  • Authors:
    • Paulina M. Wojnarowicz
    • Diane M. Provencher
    • Anne-Marie Mes-Masson
    • Patricia N. Tonin
  • Corresponding author:
  • View Affiliations

  • Published online on: Tuesday, February 14, 2012
  • Pages: 1865-1880 DOI: 10.3892/ijo.2012.1371

Abstract

It has been proposed that the frequent loss of heterozygosity (LOH) of an entire chromosome 17 contig in epithelial ovarian cancers (EOC) is the consequence of the inactivation of multiple tumour suppressor genes on this chromosome. We report the characterization of a 453 Kb 17q25 locus shown previously to exhibit a high frequency of LOH in EOC samples. LOH analysis further defined the minimal region of deletion to a 65 Kb interval flanked by D17S2239 and D17S2244, which contains RHBDF2, CYGB and PRCD as tumour suppressor gene candidates. Tissue specific expression excluded PRCD as a candidate. RHBDF2 was expressed at low levels in the majority of benign and low malignant potential (LMP) tumours, and in a subset of malignant ovarian tumour samples, as compared with primary cultures of normal ovarian surface epithelial cell (NOSE) samples. CYGB was expressed at low levels in the majority of LMP and malignant samples compared with benign and NOSE samples. In contrast to CYGB expression, RHBDF2 was expressed at low or undetectable levels in EOC cell lines exhibiting tumourigenic characteristics and up-regulated in a genetically modified EOC cell line rendered non-tumourigenic. DNA sequence analysis identified variants but no apparent deleterious mutations in either gene. Methylation-specific PCR analysis suggested that promoter methylation of CYGB but not RHBDF2 occurred in 6 of 31 malignant samples. The results combined suggest that RHBDF2 and CYGB may play distinctive roles in ovarian cancer and could be added to the growing roster of chromosome 17 genes implicated in this disease.
Journal Cover

June 2012
Volume 40 Issue 6

Print ISSN: 1019-6439
Online ISSN:1791-2423

2014 Impact Factor: 3.025
Ranked #30/211 Oncology
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APA
Wojnarowicz, P.M., Provencher, D.M., Mes-Masson, A., & Tonin, P.N. (2012). Chromosome 17q25 genes, RHBDF2 and CYGB, in ovarian cancer. International Journal of Oncology, 40, 1865-1880. http://dx.doi.org/10.3892/ijo.2012.1371
MLA
Wojnarowicz, P. M., Provencher, D. M., Mes-Masson, A., Tonin, P. N."Chromosome 17q25 genes, RHBDF2 and CYGB, in ovarian cancer". International Journal of Oncology 40.6 (2012): 1865-1880.
Chicago
Wojnarowicz, P. M., Provencher, D. M., Mes-Masson, A., Tonin, P. N."Chromosome 17q25 genes, RHBDF2 and CYGB, in ovarian cancer". International Journal of Oncology 40, no. 6 (2012): 1865-1880. http://dx.doi.org/10.3892/ijo.2012.1371