Erythropoietin combined with granulocyte colony‑stimulating factor enhances MMP-2 expression in mesenchymal stem cells and promotes cell migration

  • Authors:
    • Qiang Yu
    • Long Chen
    • Yong You
    • Can Zou
    • Youshun  Zhang
    • Qihuan Liu
    • Fanjun Cheng
  • Corresponding author:
  • View Affiliations

  • Published online on: Thursday, October 21, 2010
  • Pages: 31-36
  • DOI: 10.3892/mmr.2010.387

Abstract

Mesenchymal stem cells (MSCs) are promising clinical tools, but the molecular mechanisms that regulate the mobilization and homing of MSCs and cause invasion through extracellular matrix (ECM) barriers are unknown. Matrix metalloproteinase (MMP) degrades the ECM and promotes cell migration. In this study, we investigated MMP expression and cell migration after treatment with erythropoietin (EPO) and granulocyte colony-stimulating factor (G-CSF). Specifically, we studied whether EPO combined with G-CSF enhanced MMP expression and increased the in vitro motility of MSCs. Real-time PCR was used to detect the mRNA of MMPs and tissue inhibitors of metalloproteinase. Cell migration was evaluated by transwell and wound healing assays. Western blotting was used to detect changes in ERK1/2 protein levels. The results showed that EPO combined with G-CSF enhanced MMP-2 expression in MSCs, promoted MSC motility and activated the ERK1/2 signaling pathway. Thus, a combination treatment of EPO with G-CSF promoted cell migration by stimulating MMP-2 expression in MSCs and this appeared to be related to the ERK1/2 signaling pathway.
Journal Cover

January 2011
Volume 4 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

2013 Impact Factor: 1.484
Ranked #59/122 Medicine Research and Experimental
(total number of cites)

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APA
Yu, Q., Chen, L., You, Y., Zou, C., Zhang, Y., Liu, Q., & Cheng, F. (2011). Erythropoietin combined with granulocyte colony‑stimulating factor enhances MMP-2 expression in mesenchymal stem cells and promotes cell migration. Molecular Medicine Reports, 4, 31-36. http://dx.doi.org/10.3892/mmr.2010.387
MLA
Yu, Q., Chen, L., You, Y., Zou, C., Zhang, Y., Liu, Q., Cheng, F."Erythropoietin combined with granulocyte colony‑stimulating factor enhances MMP-2 expression in mesenchymal stem cells and promotes cell migration". Molecular Medicine Reports 4.1 (2011): 31-36.
Chicago
Yu, Q., Chen, L., You, Y., Zou, C., Zhang, Y., Liu, Q., Cheng, F."Erythropoietin combined with granulocyte colony‑stimulating factor enhances MMP-2 expression in mesenchymal stem cells and promotes cell migration". Molecular Medicine Reports 4, no. 1 (2011): 31-36. http://dx.doi.org/10.3892/mmr.2010.387