WWOX-mediated apoptosis in A549 cells mainly involves the mitochondrial pathway

  • Authors: Ping Zhang, Renbing Jia, Lei Ying, Bo Liu, Guanxiang Qian, Xianqun Fan, Shengfang Ge
  • View Affiliations

  • Published online on: Friday, April 6, 2012
  • Pages: 121-124
  • DOI: 10.3892/mmr.2012.860

Abstract

The human WWOX gene, known as WW domain-containing oxidoreductase, is located on 16q23.3-24.1, a chromosome region that spans the common fragile site, FRA16D. Abnormal transcripts or even loss of expression are frequently found in a number of cancer cell types, including breast, ovarian, prostate and lung cancer cells. It has therefore been proposed that the WWOX gene encodes a candidate tumor suppressor, possibly a pro-apoptotic protein. However, the mechanism behind this is not entirely clear. In the present study, we examined the pro-apoptotic action of WWOX using transient expression in A549 cells. We observed that the ectopic expression of WWOX caused apoptosis in A549 cells. We further observed procaspase-3 and procaspase-9 activation and the release of cytochrome C from the mitochondria in A549 cells transfected with pcDNA3.0-WWOX. These data indicate that WWOX induces apoptosis in A549 cells via the mitochondrial pathway.
Journal Cover

July 2012
Volume 6 Issue 1

Print ISSN: 1791-2997
Online ISSN:1791-3004

2013 Impact Factor: 1.484
Ranked #59/122 Medicine Research and Experimental
(total number of cites)

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