|WWOX-mediated apoptosis in A549 cells mainly involves the mitochondrial pathway|
Authors: Ping Zhang, Renbing Jia, Lei Ying, Bo Liu, Guanxiang Qian, Xianqun Fan, Shengfang Ge
Affiliations: Department of Biochemistry and Molecular Biology, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China, Department of Ophthalmology, Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China
Published online on: Friday, April 6, 2012
The human WWOX gene, known as WW domain-containing oxidoreductase, is located on 16q23.3-24.1, a chromosome region that spans the common fragile site, FRA16D. Abnormal transcripts or even loss of expression are frequently found in a number of cancer cell types, including breast, ovarian, prostate and lung cancer cells. It has therefore been proposed that the WWOX gene encodes a candidate tumor suppressor, possibly a pro-apoptotic protein. However, the mechanism behind this is not entirely clear. In the present study, we examined the pro-apoptotic action of WWOX using transient expression in A549 cells. We observed that the ectopic expression of WWOX caused apoptosis in A549 cells. We further observed procaspase-3 and procaspase-9 activation and the release of cytochrome C from the mitochondria in A549 cells transfected with pcDNA3.0-WWOX. These data indicate that WWOX induces apoptosis in A549 cells via the mitochondrial pathway.