Upregulation of nuclear factor‑κB and acid sensing ion channel 3 in dorsal root ganglion following application of nucleus pulposus onto the nerve root in rats

  • Authors:
    • Dong Wang
    • Hao Pan
    • Hang Zhu
    • Li Zhu
    • Yong‑Jiang He
    • Jian Wang
    • Gao‑Yong Jia
  • View Affiliations

  • Published online on: July 27, 2017     https://doi.org/10.3892/mmr.2017.7094
  • Pages: 4309-4314
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Abstract

The nucleus pulposus (NP) is an avascular, hydrated tissue that permits the intervertebral disc to resist compressive loads to the spine. To determine the mechanisms by which intervertebral disc degeneration is caused by the nucleus pulposus, the expression and regulation of nuclear factor (NF)‑κB and acid sensing ion channel 3 (ASIC3) were examined. For the intervertebral disc degeneration model, NP was harvested from the tail of rats and applied to the L5 dorsal root ganglion (DRG). The mechanical pain withdrawal threshold (PWT) in NP model rats was assessed. Reverse transcription‑quantitative polymerase chain reaction and western blotting were used to examine NF‑κB and ASIC3 expression levels in DRG. Finally, the effect of the NF‑κB inhibitor pyrrolidine dithiocarbamate (PDTC) and the ASIC3 signaling pathway blocker amiloride were examined. Rats exposed to NP exhibited decreased PWT for 12 days, and NF‑κB and ASIC3 was upregulated in DRG induced by NP 14 days after surgery. After administration of amiloride and PDTC to DRG affected by NP, the levels of nitric oxide (NO), tumor necrosis factor‑α (TNF‑α), interleukin‑6 (IL‑6), NF‑κB and ASIC3 were downregulated, and the levels of aquaporin (AQP) 1 and AQP3 were significantly increased for 14 days. In conclusion, these results suggested that NF‑κB and ASIC3 may serve an important role in intervertebral disc degeneration caused by NP.

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October-2017
Volume 16 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang D, Pan H, Zhu H, Zhu L, He YJ, Wang J and Jia GY: Upregulation of nuclear factor‑κB and acid sensing ion channel 3 in dorsal root ganglion following application of nucleus pulposus onto the nerve root in rats. Mol Med Rep 16: 4309-4314, 2017.
APA
Wang, D., Pan, H., Zhu, H., Zhu, L., He, Y., Wang, J., & Jia, G. (2017). Upregulation of nuclear factor‑κB and acid sensing ion channel 3 in dorsal root ganglion following application of nucleus pulposus onto the nerve root in rats. Molecular Medicine Reports, 16, 4309-4314. https://doi.org/10.3892/mmr.2017.7094
MLA
Wang, D., Pan, H., Zhu, H., Zhu, L., He, Y., Wang, J., Jia, G."Upregulation of nuclear factor‑κB and acid sensing ion channel 3 in dorsal root ganglion following application of nucleus pulposus onto the nerve root in rats". Molecular Medicine Reports 16.4 (2017): 4309-4314.
Chicago
Wang, D., Pan, H., Zhu, H., Zhu, L., He, Y., Wang, J., Jia, G."Upregulation of nuclear factor‑κB and acid sensing ion channel 3 in dorsal root ganglion following application of nucleus pulposus onto the nerve root in rats". Molecular Medicine Reports 16, no. 4 (2017): 4309-4314. https://doi.org/10.3892/mmr.2017.7094