Open Access

Expression of matrix metalloproteinase-26 promotes human glioma U251 cell invasion in vitro and in vivo

  • Authors:
    • Yiping Deng
    • Wei Li
    • Yilei Li
    • Hongfa Yang
    • Hua Xu
    • Shanshan Liang
    • Lihong Zhang
    • Yulin Li
  • View Affiliations

  • Published online on: January 1, 2010     https://doi.org/10.3892/or_00000607
  • Pages: 69-78
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Abstract

MMP-26 is a novel member of the MMP family and is widely expressed in cancer cells of epithelial origin. Published research shows that MMP-26 contributes to tumor development and to the restoration of tissue injury. In this study, in order to identify the functions of MMP-26 that contribute to the biological phenotype and behavior of non-epithelial human glioma U251cells, we established an MMP-26 overexpressing tumor cell model using gene transfection. We then used these cells to investigate the role of MMP-26 in tumor progression. Adherence and spreading assay, wound healing assay, Boyden chamber invasion assay, and in vivo tumorigenicity assay were performed to analyze the invasion ability of MMP-26 transfected U251 cells. Microvessel density analysis and tumor cell induced angiogenesis assay were employed to detect the function of MMP-26 in angiogenesis. Results showed that the spreading cell ratio of MMP-26 transfected cells was significantly higher than parental U251 cells. The relative migration distance of MMP-26 transfected cells on Matrigel was significantly higher than that of parental U251 cells. The Boyden chamber assay showed that MMP-26 could significantly enhance the ability of U251 cells to invade through Matrigel. MMP-26 could also enhance the local invasion ability of U251 cells in vivo. There was a significant increase of the microvessel density of tumor tissue derived from MMP-26 transfected U251 cells. The vessel numbe induced by MMP-26 transfected U251 cells in nude mice was also significantly higher than that induced by parental U251 cells. In conclusion, we successfully established an MMP-26 overexpressing cell model and confirmed that MMP-26 contributed to U251 cell invasion and migration in vitro. We also demonstrated that MMP-26 plays an important role in local invasion, and angiogenesis.

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January 2010
Volume 23 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Deng Y, Li W, Li Y, Yang H, Xu H, Liang S, Zhang L and Li Y: Expression of matrix metalloproteinase-26 promotes human glioma U251 cell invasion in vitro and in vivo. Oncol Rep 23: 69-78, 2010.
APA
Deng, Y., Li, W., Li, Y., Yang, H., Xu, H., Liang, S. ... Li, Y. (2010). Expression of matrix metalloproteinase-26 promotes human glioma U251 cell invasion in vitro and in vivo. Oncology Reports, 23, 69-78. https://doi.org/10.3892/or_00000607
MLA
Deng, Y., Li, W., Li, Y., Yang, H., Xu, H., Liang, S., Zhang, L., Li, Y."Expression of matrix metalloproteinase-26 promotes human glioma U251 cell invasion in vitro and in vivo". Oncology Reports 23.1 (2010): 69-78.
Chicago
Deng, Y., Li, W., Li, Y., Yang, H., Xu, H., Liang, S., Zhang, L., Li, Y."Expression of matrix metalloproteinase-26 promotes human glioma U251 cell invasion in vitro and in vivo". Oncology Reports 23, no. 1 (2010): 69-78. https://doi.org/10.3892/or_00000607