DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation

Li,Ya‑Ting; Xie,Ming‑Kun; Wu,Jin

doi:10.3892/br.2014.332

DNA profiling in peripheral blood, buccal swabs, hair follicles and semen from a patient following allogeneic hematopoietic stem cells transplantation

Authors:
- Ya‑Ting Li
- Ming‑Kun Xie
- Jin Wu
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Affiliations: Department of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, Sichuan 610041, P.R. China
Published online on: August 5, 2014 https://doi.org/10.3892/br.2014.332
Pages: 804-808

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Abstract

Allogeneic peripheral blood stem cells transplantation (allo‑PBSCT) or allogeneic bone marrow transplantation (allo‑BMT) have been widely used to treat patients exhibiting certain severe illnesses. However, previous studies have shown that the biological materials of allo‑PBSCT or allo‑BMT recipients may not constitute credible materials for personal identification. In the present study, four types of commonly used samples were collected from a male individual following gender‑matched allo‑BMT. Autosomal short tandem repeat (STR) and Y‑STR markers analysis, based on polymerase chain reaction, were used to evaluate the chimerism status. The results showed that the blood sample were all donor type, the buccal swab sample were mixed chimerism, and the sperm and hair follicle samples maintained a recipient origin of 100%. In conclusion, identical results were obtained by the two methods and it was confirmed that DNA extracted from hair follicles and sperm can be used as a reference for the pre‑transplant genotype DNA profile of the recipient in the gender‑match allo‑BMT or ‑PBSCT.

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