Association of THBS1 rs1478605 T>C in 5'-untranslated regions with the development and progression of gastric cancer

Hong,Bin-Bin; Chen,Shu-Qin; Qi,Yuan-Lin; Zhu,Jin-Wei; Lin,Jian-Yin

doi:10.3892/br.2015.414

Association of THBS1 rs1478605 T>C in 5'-untranslated regions with the development and progression of gastric cancer

Authors:
- Bin-Bin Hong
- Shu-Qin Chen
- Yuan-Lin Qi
- Jin-Wei Zhu
- Jian-Yin Lin
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Affiliations: Central Laboratory of The Second Affiliated Hospital of Fujian Medical University, Quanzhou, Fujian 362000, P.R. China, Key Laboratory of Ministry of Education for Gastrointestinal Cancer, Research Center of Molecular Medicine, Fujian Medical University, Fuzhou, Fujian 350004, P.R. China
Published online on: January 5, 2015 https://doi.org/10.3892/br.2015.414
Pages: 207-214

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Abstract

Thrombospondin 1 (THBS1) plays an important role in angiogenesis and tumor progression. The aim of the present study was to investigate the effects of single‑nucleotide polymorphisms (rs1478605 and rs3743125) in the untranslated regions of the THBS1 gene on the development and progression of gastric cancer. In the case‑control study, 275 gastric cancer patients and 275 cancer‑free controls were successfully genotyped using polymerase chain reaction‑restriction fragment length polymorphism. The data demonstrated that THBS1 rs1478605 genotypic distributions significantly differed between the patient and control groups (P=0.005). Carriers of the CC genotype exhibited a decreased risk of developing gastric cancer compared to the carriers of the CT and TT genotypes [adjusted odd ratio (OR), 0.56; 95% confidence interval (CI), 0.39‑0.79; P=0.001]. The CC genotype of rs1478605 was negatively associated with gastric cancer lymph node metastasis (OR, 0.41; 95% CI, 0.23‑0.71; P=0.001) and was associated with a reduced risk of lymph node metastasis in male patients (OR, 0.27; 95% CI, 0.14‑0.52; P<0.001). The THBS1 CT haplotype was associated with a reduced risk of developing gastric cancer (OR, 0.56; 95% CI, 0.33‑0.93; P=0.02). By contrast, no association was observed between THBS1 rs3743125 and the development and progression of gastric cancer. These results suggest that THBS1 rs1478605 represents a potential molecular marker for gastric cancer.

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