p38 mitogen-activated protein kinase/activator protein-1 involved in serum deprivation-induced human alkaline ceramidase 2 upregulation

Huang,Zhaoquan; Huang,Guojin; Li,Quanzhong; Jin,Junfei

doi:10.3892/br.2014.394

p38 mitogen-activated protein kinase/activator protein-1 involved in serum deprivation-induced human alkaline ceramidase 2 upregulation

Authors:
- Zhaoquan Huang
- Guojin Huang
- Quanzhong Li
- Junfei Jin
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Affiliations: Department of Pathology, Guilin Medical University, Guilin, Guangxi 541001, P.R. China, Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China, Department of Cardiology, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541001, P.R. China
Published online on: December 2, 2014 https://doi.org/10.3892/br.2014.394
Pages: 225-229

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Abstract

Our previous study revealed that serum deprivation upregulated human alkaline ceramidase 2 (haCER2) activity and mRNA in HeLa cells, but the mechanism remains unknown. In the present study, serum deprivation also upregulated haCER2 activity in HepG2 human hepatoma cell line cells due to an increase in haCER2 mRNA, in which mRNA transcription, not mRNA stability, is involved. Furthermore, p38 mitogen‑activated protein kinase (MAPK)/activator protein‑1 (AP‑1) signaling pathway is involved in haCER2 mRNA upregulation by serum deprivation, and this mechanism may explain why haCER2 is upregulated in human liver cancer. In conclusion, p38 MAPK, AP‑1 or haCER2 may be used as targets in liver cancer therapy.

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