Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation

Ju,Rong; Zeng,Wen; Wu,Rong; Feng,Zhichun

doi:10.3892/br.2014.405

Interaction between neural stem cells and bone marrow derived-mesenchymal stem cells during differentiation

Authors:
- Rong Ju
- Wen Zeng
- Rong Wu
- Zhichun Feng
View Affiliations

Affiliations: Department of Neonatology, Chengdu Women's and Children's Central Hospital, Chongqing Medical University, Chengdu, Sichuan 610091, P.R. China, Neonatal Medical Center, Huaian Maternity and Child Healthcare Hospital Affiliated to Yangzhou University Medical Academy, Huaian, Jiangsu 223002, P.R. China, Department of Neonatology, Bayi Children's Hospital Affiliated to General Hospital of Beijing Military Command, Beijing 100700, P.R. China
Published online on: December 17, 2014 https://doi.org/10.3892/br.2014.405
Pages: 242-246

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Due to their capacity to self‑replicate or produce specific differentiated cell types, neural stem cells (NSCs) and bone marrow derived‑mesenchymal stem cells (BMSCs) are potential sources for cell transplantation therapies, particularly for neural injury. However, the interaction between NSCs and BMSCs during differentiation has not yet been defined. The interaction is believed to improve the effectiveness and efficiency of cell therapy. In the present study, human NSCs and BMSCs were cultured and the Transwell co‑culture system was used to observe the interplay between NSCs and BMSCs during differentiation. The results revealed that NSCs promoted BMSCs to differentiate into neurons and NSCs; whereas, BMSCs did not affect the differentiation of NSCs. Simultaneously, co‑culture increased the concentration of brain‑derived neurotrophic factor (BDNF) and nerve growth factor (NGF), which are secreted by NSCs and BMSCs. The present findings suggest that co‑culture of NSCs and BMSCs can promote the differentiation and this process may be modulated by BDNF and NGF.

View Figures

View References

1	Hess DC and Borlongan CV: Stem cells and neurological diseases. Cell Prolif. 41 (Suppl 1):94–114. 2008. View Article : Google Scholar : PubMed/NCBI
2	Song H, Stevens CF and Gage FH: Astroglia induce neurogenesis from adult neural stem cells. Nature. 417:39–44. 2002. View Article : Google Scholar : PubMed/NCBI
3	Johansson CB, Momma S, Clarke DL, Risling M, Lendahl U and Frisen J: Identification of a neural stem cell in the adult mammalian central nervous system. Cell. 96:25–34. 1999. View Article : Google Scholar : PubMed/NCBI
4	Temple S: The development of neural stem cells. Nature. 414:112–117. 2001. View Article : Google Scholar : PubMed/NCBI
5	Woodbury D, Schwarz EJ, Prockop DJ and Black IB: Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res. 61:364–370. 2000. View Article : Google Scholar : PubMed/NCBI
6	Hermann A, Gastl R, Liebau S, et al: Efficient generation of neural stem cell-like cells from adult human bone marrow stromal cells. J Cell Sci. 117:4411–4422. 2004. View Article : Google Scholar : PubMed/NCBI
7	Lin W, Chen X, Wang X, Liu J and Gu X: Adult rat bone marrow stromal cells differentiate into Schwann cell-like cells in vitro. In Vitro Cell Dev Biol Anim. 44:31–40. 2008. View Article : Google Scholar : PubMed/NCBI
8	Zhao LR, Duan WM, Reyes M, Keene CD, Verfaillie CM and Low WC: Human bone marrow stem cells exhibit neural phenotypes and ameliorate neurological deficits after grafting into the ischemic brain of rats. Exp Neurol. 174:11–20. 2002. View Article : Google Scholar : PubMed/NCBI
9	Novikova LN, Brohlin M, Kingham PJ, Novikov LN and Wiberg M: Neuroprotective and growth-promoting effects of bone marrow stromal cells after cervical spinal cord injury in adult rats. Cytotherapy. 13:873–887. 2011. View Article : Google Scholar : PubMed/NCBI
10	Hawryluk GW, Mothe A, Wang J, Wang S, Tator C and Fehlings MG: An in vivo characterization of trophic factor production following neural precursor cell or bone marrow stromal cell transplantation for spinal cord injury. Stem Cells Dev. 21:2222–2238. 2012. View Article : Google Scholar : PubMed/NCBI
11	Hamada H, Kobune M, Nakamura K, et al: Mesenchymal stem cells (MSC) as therapeutic cytoreagents for gene therapy. Cancer Sci. 96:149–156. 2005. View Article : Google Scholar : PubMed/NCBI
12	El-Badri NS, Hakki A, Saporta S, et al: Cord blood mesenchymal stem cells: Potential use in neurological disorders. Stem Cells Dev. 15:497–506. 2006. View Article : Google Scholar : PubMed/NCBI
13	Flax JD, Aurora S, Yang C, et al: Engraftable human neural stem cells respond to development cues, replace neurons, and express foreign genes. Nat Biotechnol. 16:1033–1039. 1998. View Article : Google Scholar : PubMed/NCBI
14	Kafienah W, Mistry S, Williams C and Hollander AP: Nucleostemin is a marker of proliferating stromal stem cells in adult human bone marrow. Stem Cells. 24:1113–1120. 2006. View Article : Google Scholar : PubMed/NCBI
15	Chen JR, Cheng GY, Sheu CC, Tseng GF, Wang TJ and Huang YS: Transplanted bone marrow stromal cells migrate, differentiate and improve motor function in rats with experimentally induced cerebral stroke. J Anat. 213:249–258. 2008. View Article : Google Scholar : PubMed/NCBI
16	Chu K, Kim M, Park KI, et al: Human neural stem cells improve sensorimotor deficits in the adult rat brain with experimental focal ischemia. Brain Res. 1016:145–153. 2004. View Article : Google Scholar : PubMed/NCBI
17	Crigler L, Robey RC, Asawachaicharn A, Gaupp D and Phinney DG: Human mesenchymal stem cell subpopulations express a variety of neuro-regulatory molecules and promote neuronal cell survival and neuritogenesis. Exp Neurol. 198:54–64. 2006. View Article : Google Scholar : PubMed/NCBI
18	Llado J, Haenggeli C, Maragakis NJ, Snyder EY and Rothstein JD: Neural stem cells protect against glutamate-induced excitotoxicity and promote survival of injured motor neurons through the secretion of neurotrophic factors. Mol Cell Neurosci. 27:322–331. 2004. View Article : Google Scholar : PubMed/NCBI
19	Piirsoo M, Kaljas A, Tamm K and Timmusk T: Expression of NGF and GDNF family members and their receptors during peripheral nerve development and differentiation of Schwann cells in vitro. Neurosci Lett. 469:135–140. 2010. View Article : Google Scholar : PubMed/NCBI
20	Razavi S, Razavi MR, Kheirollahi-Kouhestani M, Mardani M and Mostafavi FS: Co-culture with neurotrophic factor secreting cells induced from adipose-derived stem cells: Promotes neurogenic differentiation. Biochem Biophys Res Commun. 440:381–387. 2013. View Article : Google Scholar : PubMed/NCBI
21	Yuan J, Huang G, Xiao Z, Lin L and Han T: Overexpression of β-NGF promotes differentiation of bone marrow mesenchymal stem cells into neurons through regulation of AKT and MAPK pathway. Mol Cell Biochem. 383:201–211. 2013. View Article : Google Scholar : PubMed/NCBI
22	Deng YB, Ye WB, Hu ZZ, et al: Intravenously administered BMSCs reduce neuronal apoptosis and promote neuronal proliferation through the release of VEGF after stroke in rats. Neurol Res. 32:148–156. 2010. View Article : Google Scholar : PubMed/NCBI
23	Shen CC, Lin CH, Yang YC, Chiao MT, Cheng WY and Ko JL: Intravenous implanted neural stem cells migrate to injury site, reduce infarct volume, and improve behavior after cerebral ischemia. Curr Neurovasc Res. 7:167–179. 2010. View Article : Google Scholar : PubMed/NCBI
24	Walker PA, Letourneau PA, Bedi S, Shah SK, Jimenez F and Cox CS Jr: Progenitor cells as remote ‘bioreactors’: Neuroprotection via modulation of the systemic inflammatory response. World J Stem Cells. 3:9–18. 2011. View Article : Google Scholar : PubMed/NCBI
25	Hokari M, Kuroda S, Shichinohe H, Yano S, Hida K and Iwasaki Y: Bone marrow stromal cells protect and repair damaged neurons through multiple mechanisms. J Neurosci Res. 86:1024–1035. 2008. View Article : Google Scholar : PubMed/NCBI
26	Walker PA, Harting MT, Jimenez F, et al: Direct intrathecal implantation of mesenchymal stromal cells leads to enhanced neuroprotection via an NFkabba B-mediated increase in interleukin-6 production. Stem Cells Dev. 19:867–876. 2010. View Article : Google Scholar : PubMed/NCBI
27	Sanchez-Ramos J, Song S, Cardozo-Pelaez F, et al: Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol. 164:247–256. 2000. View Article : Google Scholar : PubMed/NCBI
28	Lou SJ, Gu P, Chen F, He C, Wang MW and Lu CL: The effect of bone marrow stromal cells on neuronal differentiation of mesencephalic neural stem cells in Sprague-Dawley rats. Brain Res. 968:114–121. 2003. View Article : Google Scholar : PubMed/NCBI
29	Wang Y, Tu W, Lou Y, et al: Mesenchymal stem cells regulate the proliferation and differentiation of neural stem cells through Notch signaling. Cell Biol Int. 33:1173–1179. 2009. View Article : Google Scholar : PubMed/NCBI
30	Chen BY, Wang X, Wang ZY, Wang YZ, Chen LW and Luo ZJ: Brain-derived neurotrophic factor stimulates proliferation and differentiation of neural stem cells, possibly by triggering the Wnt/β-catenin signaling pathway. J Neurosci Res. 91:30–41. 2013.PubMed/NCBI