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Realgar quantum dots induce apoptosis and necrosis in HepG2 cells through endoplasmic reticulum stress

  • Authors:
    • Yu Qin
    • Huan Wang
    • Zheng‑Yun Liu
    • Jie Liu
    • Jin‑Zhu Wu

  • View Affiliations

  • Published online on: July 15, 2015     https://doi.org/10.3892/br.2015.489
  • Pages: 657-662
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Abstract

Realgar (As4S4) has been used in traditional Chinese medicines for treatment of malignancies. However, the poor water solubility of realgar limits its clinical application. To overcome this problem, realgar quantum dots (RQDs; 5.48±1.09 nm) were prepared by a photoluminescence method. The mean particle size was characterized by high‑resolution transmission electron microscopy and scanning electron microscopy. Our recent studies revealed that the RQDs were effective against tumor growth in tumor‑bearing mice without producing apparent toxicity. The present study investigated their anticancer effects and mechanisms in human hepatocellular carcinoma (HepG2) cells. The HepG2 cells and human normal liver (L02) cells were used to determine the cytotoxicity of RQDs. The portion of apoptotic and dead cells were measured by flow cytometry with Annexin V‑fluorescein isothiocyanate/propidium iodide double staining. Apoptosis‑related proteins and genes were examined by western blot analysis and reverse transcription‑quantitative polymerase chain reaction, and the mitochondrial membrane potential was assayed by confocal microscope with JC‑1 as a probe. RQDs exhibited cytotoxicity in a concentration‑dependent manner and HepG2 cells were more sensitive compared with normal L02 cells. At 15 µg/ml, 20% of the cells were apoptotic, while 60% of the cells were necrotic at 30 µg/ml. The anti‑apoptosis protein Bcl‑2 was dose‑dependently decreased, while pro‑apoptotic protein Bax was increased. There was a loss of mitochondrial membrane potential and expression of the stress genes C/EBP‑homologous protein 10 and glucose‑regulated protein 78 was increased by RQDs. RQDs were effective in the inhibition of HepG2 cell proliferation and this effect was due to induction of apoptosis and necrosis through endoplasmic reticulum stress.
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September-2015
Volume 3 Issue 5

Print ISSN: 2049-9434
Online ISSN:2049-9442

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  • Journal Metrics
  • Impact Factor: 2.3
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  • CiteScore: 3.7
  • CiteScore Rank: Q2: #23/79 Pharmacology, Toxicology and Pharmaceutics
  • Source Normalized Impact per Paper (SNIP): 0.742
  • SCImago Journal Rank (SJR): 0.488
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Spandidos Publications style
Qin Y, Wang H, Liu ZY, Liu J and Wu JZ: Realgar quantum dots induce apoptosis and necrosis in HepG2 cells through endoplasmic reticulum stress. Biomed Rep 3: 657-662, 2015
APA
Qin, Y., Wang, H., Liu, Z., Liu, J., & Wu, J. (2015). Realgar quantum dots induce apoptosis and necrosis in HepG2 cells through endoplasmic reticulum stress. Biomedical Reports, 3, 657-662. https://doi.org/10.3892/br.2015.489
MLA
Qin, Y., Wang, H., Liu, Z., Liu, J., Wu, J."Realgar quantum dots induce apoptosis and necrosis in HepG2 cells through endoplasmic reticulum stress". Biomedical Reports 3.5 (2015): 657-662.
Chicago
Qin, Y., Wang, H., Liu, Z., Liu, J., Wu, J."Realgar quantum dots induce apoptosis and necrosis in HepG2 cells through endoplasmic reticulum stress". Biomedical Reports 3, no. 5 (2015): 657-662. https://doi.org/10.3892/br.2015.489