Anti-allergic effect of the naturally-occurring conjugated linolenic acid isomer, jacaric acid, on the activated human mast cell line-1

Liu,Wai  Nam; Leung,Kwok  Nam

doi:10.3892/br.2015.517

Anti-allergic effect of the naturally-occurring conjugated linolenic acid isomer, jacaric acid, on the activated human mast cell line-1

Authors:
- Wai Nam Liu
- Kwok Nam Leung
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Affiliations: Biochemistry Programme, School of Life Sciences, The Chinese University of Hong Kong, Shatin, Hong Kong, SAR, P.R. China
Published online on: September 24, 2015 https://doi.org/10.3892/br.2015.517
Pages: 839-842

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Abstract

The present study aimed to investigate the immunomodulatory effect of jacaric acid, a naturally‑occurring conjugated linolenic acid isomer that can be found in jacaranda seed oil, on the activated human mast cell line-1 (HMC-1). Our previous studies have demonstrated that jacaric acid only exerted minimal, if any, cytotoxicity on normal murine cells. In the present study, jacaric acid at concentrations ≤100 µM did not exhibit direct cytotoxicity on human peripheral blood mononuclear cells after 72 h of incubation, as determined by the MTT reduction assay. By contrast, jacaric acid could alleviate the calcium ionophore A23187 and phorbol 12‑myristate 13‑acetate‑triggered allergic response in the HMC‑1 cells at concentrations that were non‑cytotoxic to the HMC‑1 cells. Following pre‑treatment with jacaric acid, the secretion of two inflammatory mediators, β‑N‑acetylglucosaminidase and tryptase, as well as the T helper 2 cytokines [interleukin (IL)‑4 and IL‑13] was significantly reduced in HMC‑1 cells. The alleviation of allergic response was accompanied by downregulation of the matrix metalloproteinase‑2 and ‑9 proteins and upregulation of the tissue inhibitor of metalloproteinase‑1 protein. Collectively, the results indicated that the naturally‑occurring jacaric acid exhibits a suppressive effect on the allergic response in activated human mast cells in vitro, and this could not be attributed to the direct cytotoxicity of jacaric acid on the treated cells.

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