V‑PYRRO/NO downregulates mRNA expression levels of leukotriene C4 synthase during hepatic ischemia reperfusion injury in rats via inhibition of the nuclear factor‑κB activation pathway

Hong,Feng‑Fang; Wang,Yi‑Fan; Liu,Hui; Yang,Mei‑Wen; Yang,Shu‑Long

doi:10.3892/br.2015.533

V‑PYRRO/NO downregulates mRNA expression levels of leukotriene C4 synthase during hepatic ischemia reperfusion injury in rats via inhibition of the nuclear factor‑κB activation pathway

Authors:
- Feng‑Fang Hong
- Yi‑Fan Wang
- Hui Liu
- Mei‑Wen Yang
- Shu‑Long Yang
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Affiliations: Department of Physiology, College of Medicine, Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Institute of Cancer Research, Jiangxi Academy of Medical Science, Nanchang, Jiangxi 330006, P.R. China, Fuzhou Medical College, Nanchang University, Fuzhou 344000, P.R. China
Published online on: October 16, 2015 https://doi.org/10.3892/br.2015.533
Pages: 112-116

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Abstract

The aim of the present study was to explore the mechanism underlying the effects of a selective liver nitric oxide (NO) donor, O2-vinyl1-(pyrrolidin-1-yl)-diazen-1-ium-1,2-diolate (V-PYRRO/NO), on the gene expression of leukotriene C4 synthase (LTC4S) during hepatic ischemia/reperfusion (I/R). Adult male Sprague-Dawley rats were divided into 3 groups: Sham (control), I/R and V-PYRRO/NO + I/R groups. The liver was subjected to 1 h of partial hepatic ischemia followed by 5 h of reperfusion, saline or V-PYRRO/NO (1.06 µmol/kg/h) administered intravenously. The mRNA expression levels of LTC4S in rat liver tissue were examined by the reverse transcription-polymerase chain reaction method, the protein expression levels of nuclear factor-κB (NF-κB) p65, p50 and IκBα in liver cell lysates and nuclear extracts were detected by western blot analysis. Hepatic mRNA expression of LTC4S was lower in V-PYRRO/NO + I/R group compared to the I/R group. In addition, the protein expression levels of NF-κB p65 and p50 in the nucleus extract were lower in the V-PYRRO/NO + I/R group when compared with the I/R group. However, the IκBα protein in the 3 groups was not changed. Immunohistochemistry staining revealed that the I/R liver exhibited strong cytoplasmic and nuclear staining for NF-κB p65; however, the V-PYRRO/NO + I/R group liver presented slight cytoplasmic and nuclear staining. In conclusion, V-PYRRO/NO may downregulate LTC4S mRNA expression by inhibiting NF-κB activation independent of IκBα during hepatic I/R injury.

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