Effect of 2,4,6‑trimethyl‑N‑[3‑(trifluoromethyl)phenyl]benzene-sulfonamide on calcium influx in three contraction models

Grześk,Elżbieta; Szadujkis‑Szadurska,Katarzyna; Wiciński,Michał; Malinowski,Bartosz; Sinjab,Thabit A.; Tejza,Barbara; Pujanek,Maciej; Janiszewska,Ewa; Kopczyńska,Anna; Grześk,Grzegorz

doi:10.3892/br.2015.543

Effect of 2,4,6‑trimethyl‑N‑[3‑(trifluoromethyl)phenyl]benzene-sulfonamide on calcium influx in three contraction models

Authors:
- Elżbieta Grześk
- Katarzyna Szadujkis‑Szadurska
- Michał Wiciński
- Bartosz Malinowski
- Thabit A. Sinjab
- Barbara Tejza
- Maciej Pujanek
- Ewa Janiszewska
- Anna Kopczyńska
- Grzegorz Grześk
View Affiliations

Affiliations: Department of Pediatrics, Hematology and Oncology, Collegium Medicum, Nicolaus Copernicus University, 85‑094 Bydgoszcz, Poland, Department of Pharmacology and Therapeutics, Faculty of Medicine, Collegium Medicum, Nicolaus Copernicus University, 85‑094 Bydgoszcz, Poland
Published online on: November 10, 2015 https://doi.org/10.3892/br.2015.543
Pages: 117-121

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

2,4,6‑Trimethyl‑N‑[3‑(trifluoromethyl)phenyl]benzenesulfonamide (m‑3M3FBS) activates phospholipase C and stimulates apoptosis; however, in smooth muscle cells it may increase the perfusion pressure. The main aim of the present study was to evaluate the physiological effect of direct stimulation of phospholipase C on vascular smooth muscle reactivity using three contraction models. Experiments were performed on the isolated and perfused tail artery of Wistar rats. The contraction force in the present model was measured by an increased level of perfusion pressure with a constant flow. Concentration‑response curves (CRCs) obtained for phenylephrine, arg‑vasopressin, mastoparan‑7 and Bay K8644 presented a sigmoidal association. In comparison to the control curves, CRCs in the presence of m‑3M3FBS were significantly shifted to the left except for Bay K8644. Analyses of calcium influx suggest that in the presence of m‑3M3FBS the calcium influx from intra‑ and extracellular calcium stores was significantly higher. The results of the present experiments suggest that m‑3M3FBS significantly increases the reactivity of vascular smooth muscle stimulated with metabotropic receptors or G‑protein by an increase in calcium influx from intra‑ and extracellular calcium stores. The current knowledge regarding the apoptotic pathway shows the significance of calcium ions involved in this process, thus, m‑3M3FBS may induce apoptosis by an increase of cytoplasmic calcium concentration; however, simultaneously, the use of this mechanism in therapy must be preceded by a molecular modification that eliminates a possible vasoconstriction effect.

View Figures

View References

1	Rhee SG: Regulation of phosphoinositide-specific phospholipase C. Annu Rev Biochem. 70:281–312. 2001. View Article : Google Scholar : PubMed/NCBI
2	Suh BC and Hille B: Regulation of ion channels by phosphatidylinositol 4,5,-bisphosphate. Curr Opin Neurobiol. 15:370–378. 2005. View Article : Google Scholar : PubMed/NCBI
3	Lemmon MA, Ferguson KM, O'Brien R, Sigler PB and Schlessinger J: Specific and high-affinity binding of inositol phosphates to an isolated pleckstrin homology domain. Proc Natl Acad Sci USA. 92:10472–10476. 1995. View Article : Google Scholar : PubMed/NCBI
4	LaBelle EF and Polyák F: Phospholipase C beta 2 in vascular smooth muscle. J Cell Physiol. 169:358–363. 1996. View Article : Google Scholar : PubMed/NCBI
5	LaBelle EF, Wilson K and Polyák E: Subcellular localization of phospholipase C isoforms in vascular smooth muscle. Biochim Biophys Acta. 1583:273–278. 2002. View Article : Google Scholar : PubMed/NCBI
6	Horowitz LF, Hirdes W, Suh BC, Hilgemann DW, Mackie K and Hille B: Phospholipase C in living cells: Activation, inhibition, Ca²⁺ requirement and regulation of M current. J Gen Physiol. 126:243–262. 2005. View Article : Google Scholar : PubMed/NCBI
7	Powis G, Seewald MJ, Gratas C, Melder D, Riebow J and Modest EJ: Selective inhibition of phosphatidylinositol phospholipase C by cytotoxic ether lipid analogues. Cancer Res. 52:2835–2840. 1992.PubMed/NCBI
8	Smith RJ, Sam LM, Justen JM, Bundy GL, Bala GA and Bleasdale JE: Receptor-coupled signal transduction in human polymorphonuclear neutrophils: Effects of a novel inhibitor of phospholipase C-dependent processes on cell responsiveness. J Pharmacol Exp Ther. 253:688–697. 1990.PubMed/NCBI
9	Macrez-Lepretre N, Morel JL and Mironneau J: Effects of phospholipase C inhibitors on Ca2+ channel stimulation and Ca²⁺ release from intracellular stores evoked by alpha 1A- and alpha 2A-adrenoceptors in rat portal vein myocytes. Biochem Biophys Res Commun. 218:30–34. 1996. View Article : Google Scholar : PubMed/NCBI
10	Grześk E, Tejza B, Wiciński M, Malinowski B, Szadujkis-Szadurska K, Baran L, Kowal E and Grześk G: Effect of pertussis toxin on calcium influx in three contraction models. Biomed Rep. 2:584–588. 2014.PubMed/NCBI
11	Bae YS, Lee TG, Park JC, Hur JH, Kim Y, Heo K, Kwak JY, Suh PG and Ryu SH: Identification of a compound that directly stimulates phospholipase C activity. Mol Pharmacol. 63:1043–1050. 2003. View Article : Google Scholar : PubMed/NCBI
12	Szebenyi SA, Ogura T, Sathyanesan A, AlMatrouk AK, Chang J and Lin W: Increases in intracellular calcium via activation of potentially multiple phospholipase C isozymes in mouse olfactory neurons. Front Cell Neurosci. 8:3362014. View Article : Google Scholar : PubMed/NCBI
13	Krjukova J, Holmqvist T, Danis AS, Akerman KE and Kukkonen JP: Phospholipase C activator m-3M3FBS affects Ca²⁺ homeostasis independently of phospholipase C activation. Br J Pharmacol. 143:3–7. 2004. View Article : Google Scholar : PubMed/NCBI
14	Clapp TR, Medler KF, Damak S, Margolskee RF and Kinnamon SC: Mouse taste cells with G protein-coupled taste receptors lack voltage-gated calcium channels and SNAP-25. BMC Biol. 4:72006. View Article : Google Scholar : PubMed/NCBI
15	Lee YN, Lee HY, Kim JS, Park C, Choi YH, Lee TG, Ryu SH, Kwak JY and Bae YS: The novel phospholipase C activator, m-3M3FBS, induces monocytic leukemia cell apoptosis. Cancer Lett. 222:227–235. 2005. View Article : Google Scholar : PubMed/NCBI
16	Chen WC, Chou CT, Liou WC, Liu SI, Lin KL, Lu T, Lu YC, Hsu SS, Tsai JY, Liao WC, et al: Rise of [Ca²⁺]i and apoptosis induced by M-3M3FBS in SCM1 human gastric cancer cells. Chin J Physiol. 57:31–40. 2014. View Article : Google Scholar : PubMed/NCBI
17	Liu SI, Lin KL, Lu T, Lu YC, Hsu SS, Tsai JY, Liao WC, Huang FD, Chi CC, Liang WZ, et al: M-3M3FBS-induced Ca²⁺ movement and apoptosis in HA59T human hepatoma cells. Chin J Physiol. 56:26–35. 2013. View Article : Google Scholar : PubMed/NCBI
18	Grześk G: Modulujące działanie aktywatora fosfolipazy C na reakcje naczyń poddanych działaniu lipopolisacharydów, nitroprusydku sodowego i 8Br-cGMP. Bydgoszcz. UMK CM. 2008.(In Polish).
19	Kim SD, Kim HJ, Shim JW, Lee HY, Lee SK, Kwon S, Jung YS, Baek SH, Park JS, Zabel BA and Bae YS: Phospholipase C activator m-3M3FBS protects against morbidity and mortality associated with sepsis. J Immunol. 189:2000–2005. 2012. View Article : Google Scholar : PubMed/NCBI
20	Grześk G, Wiciński M, Malinowski B, Grześk E, Manysiak S, Odrowąż-Sypniewska G, Darvish N and Bierwagen M: Calcium blockers inhibits cyclosporine A-induced hyperreactivity of vascular smooth muscle cells. Mol Med Report. 5:1469–1474. 2012.
21	Grześk G and Szadujkis-Szadurski L: Pharmacometric analysis of alpha1-adrenoceptor function in rat tail artery pretreated with lipopolysaccharides. Pol J Pharmacol. 53:605–613. 2001.PubMed/NCBI
22	Grześk G and Szadujkis-Szadurski L: Physiological antagonism of angiotensin II and lipopolysaccharides in early endotoxemia: Pharmacometric analysis. Pol J Pharmacol. 55:753–762. 2003.PubMed/NCBI
23	Kawanabe Y, Hashimoto N and Masaki T: Characterization of G proteins involved in activation of nonselective cation channels and arachidonic acid release by norepinephrine/alpha1A-adrenergic receptors. Am J Physiol Cell Physiol. 286:C596–C600. 2004. View Article : Google Scholar : PubMed/NCBI
24	Chen X, Talley EM, Patel N, Gomis A, McIntire WE, Dong B, Viana F, Garrison JC and Bayliss DA: Inhibition of a background potassium channel by Gq protein alpha-subunits. Proc Natl Acad Sci USA. 103:3422–3427. 2006. View Article : Google Scholar : PubMed/NCBI
25	Kawanabe Y, Masaki T and Hashimoto N: Involvement of phospholipase C in endothelin 1-induced stimulation of Ca⁺⁺ channels and basilar artery contraction in rabbits. J Neurosurg. 105:288–293. 2006. View Article : Google Scholar : PubMed/NCBI
26	Jackson EK, Gillespie DG and Jackson TC: Phospholipase C and Src modulate angiotensin II-induced cyclic AMP production in preglomerular microvascular smooth-muscle cells from spontaneously hypertensive rats. J Cardiovasc Pharmacol. 49:106–110. 2007. View Article : Google Scholar : PubMed/NCBI
27	Szadujkis-Szadurska K, Grzesk G, Szadujkis-Szadurski L, Gajdus M and Matusiak G: Role of acetylcholine and calcium ions in three vascular contraction models: Angiotensin II, phenylephrine and caffeine. Exp Ther Med. 4:329–333. 2012.PubMed/NCBI
28	Słupski M, Szadujkis-Szadurski L, Grześk G, Szadujkis-Szadurski R, Szadujkis-Szadurska K, Wlodarczyk Z, Masztalerz M, Piotrowiak I and Jasiński M: Guanylate cyclase activators influence reactivity of human mesenteric superior arteries retrieved and preserved in the same conditions as transplanted kidneys. Transplant Proc. 39:1350–1353. 2007. View Article : Google Scholar : PubMed/NCBI
29	Jansen S, Arning J, Kemken D, Dülcks T and Beyersmann D: Phospholipase C activator 2,4,6-trimethyl-N-(meta-3-trifluoromethyl-phenyl)-benzene-sulfonamide decays under ultraviolet light and shows strong self-fluorescence. Anal Biochem. 330:353–355. 2004. View Article : Google Scholar : PubMed/NCBI
30	Jansen S, Arning J and Beyersmann D: Zinc homeostasis in C6 glioma cells: phospholipase C activity regulates cellular zinc export. Biol Trace Elem Res. 108:87–104. 2005. View Article : Google Scholar : PubMed/NCBI