Study of cyclooxygenase-2 in renal cell carcinoma

  • Authors: Rikio Yoshimura, Masahide Matsuyama, Yutaka Kawahito, Kenji Tsuchida, Katsuyuki Kuratsukuri, Yoshiaki Takemoto, Makoto Mitsuhashi, Hajime Sano, Tatsuya Nakatani
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  • Published online on: Sunday, February 1, 2004
  • Pages: 229-233
  • DOI: 10.3892/ijmm.13.2.229

Abstract

Cyclooxygenase (COX)-2 plays an important role in the development of various cancers due to its angiogenetic function. As the expression of COX-2 is up-regulated in human colorectal carcinoma and other cancers, we investigated the expression of COX-2 in human renal cell carcinoma (RCC) tissues. One hundred and eight specimens were obtained from patients with RCC, and 20 from normal kidney (NK) tissues. Immunohistochemistry, using affinity purified anti-bodies against human COX-2, and RT-PCR to study the COX-2 mRNA expression were carried out. We also examined whether or not there was a significant difference in the expression of COX-2 among grades (G1, G2, G3) and stages (pT1, pT2, pT3a, pT3b) in RCC. While no marked expression of COX-2 was observed in the NK tissues, a significantly strong expression of COX-2 was detected in RCC tissues. The extent and intensity of immunoreactive COX-2 polypeptides in cancer cells were statistically greater than those of cells from normal kidney tissues. However, no marked difference was seen among grades or between stages. These results demonstrate that the generated COX-2 in human renal cell carcinoma plays an important role in the proliferation of malignant renal cells.
Journal Cover

February 2004
Volume 13 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.957
Ranked #26/121 Medicine Research and Experimental
(total number of cites)

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APA
Yoshimura, R., Matsuyama, M., Kawahito, Y., Tsuchida, K., Kuratsukuri, K., Takemoto, Y., Mitsuhashi, M., Sano, H., & Nakatani, T. (2004). Study of cyclooxygenase-2 in renal cell carcinoma. International Journal of Molecular Medicine, 13(2), 229-233.
MLA
Yoshimura, Matsuyama, Kawahito, Tsuchida, Kuratsukuri, Takemoto, Mitsuhashi, Sano, and Tatsuya Nakatani. "Study of cyclooxygenase-2 in renal cell carcinoma." International Journal of Molecular Medicine International Journal of Molecular Medicine 13.2 (2004): 229-233.
Chicago
Yoshimura, Matsuyama, Kawahito, Tsuchida, Kuratsukuri, Takemoto, Mitsuhashi, Sano, and Tatsuya Nakatani. "Study of cyclooxygenase-2 in renal cell carcinoma." International Journal of Molecular Medicine International Journal of Molecular Medicine 13 no. 2 (2004): 229-233.