Helicobacter pylori induces chronic active gastritis in p53-knockout mice

  • Authors:
    • Junko Nagata
    • Hiroshi Kijima
    • Atsushi Takagi
    • Mamoru Ito
    • Kazuo Goto
    • Hitoshi Yamazaki
    • Masato Nakamura
    • Tetsuya Mine
    • Yoshito Ueyama
  • View Affiliations

  • Published online on: Tuesday, June 1, 2004
  • Pages: 773-777
  • DOI: 10.3892/ijmm.13.6.773

Abstract

Gastritis and peptic ulcers result from Helicobacter pylori (H. pylori) infection. To analyze the influence of Helicobacter on inflammatory responses and cell proliferation, we used an animal model of H. pylori-induced gastritis in p53-knockout mice. H. pylori were introduced by gastric intubation into p53-knockout C57BL/6 mice. The animals were then followed-up for 1 year and compared with uninfected controls of the same genotype. Serum levels of anti-H. pylori antibody and histopathological changes were analyzed according to the updated Sydney System. Immunohistochemistry for proliferating cell nuclear antigen (PCNA) and TUNEL staining were also performed. The infected mice showed significantly increased levels of anti-H. pylori antibody in serum. Histologically, p53-knockout mice exhibited increased scores of chronic and active inflammation compared with uninfected controls. The PCNA and TUNEL indices were 25.5% and 10/mm, respectively, in the inflammatory foci of infected mice, and were increased compared with the controls. In the p53-knockout mice, H. pylori infection caused severe inflammatory reactions. The p53 gene may play an important role in inflammatory responses including cell proliferation and apoptosis.
Journal Cover

June 2004
Volume 13 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Nagata, J., Kijima, H., Takagi, A., Ito, M., Goto, K., Yamazaki, H., Nakamura, M., Mine, T., & Ueyama, Y. (2004). Helicobacter pylori induces chronic active gastritis in p53-knockout mice. International Journal of Molecular Medicine, 13(6), 773-777.
MLA
Nagata, Kijima, Takagi, Ito, Goto, Yamazaki, Nakamura, Mine, and Yoshito Ueyama. "Helicobacter pylori induces chronic active gastritis in p53-knockout mice." International Journal of Molecular Medicine International Journal of Molecular Medicine 13.6 (2004): 773-777.
Chicago
Nagata, Kijima, Takagi, Ito, Goto, Yamazaki, Nakamura, Mine, and Yoshito Ueyama. "Helicobacter pylori induces chronic active gastritis in p53-knockout mice." International Journal of Molecular Medicine International Journal of Molecular Medicine 13 no. 6 (2004): 773-777.