Functional analysis of the effect of forced activation of STAT3 on M1 mouse leukemia cells

  • Authors: Tsunehiko Yoshida, Takashi Iwamoto, Koichi Adachi, Takashi Yokota, Yozo Miyake, Michinari Hamaguchi
  • View Affiliations

  • Published online on: Tuesday, February 1, 2005
  • Pages: 269-275
  • DOI: 10.3892/ijmm.15.2.269

Abstract

M1 mouse myeloid leukemia cells exhibit growth arrest and differentiation to monocytes/macrophages in response to leukemia inhibitory factor (LIF) stimulation. Although recent studies have demonstrated that STAT3 plays a central role in this process, it is unknown whether STAT3 activation alone is sufficient. To address this issue, we have established M1/STAT3ER cells, where STAT3 is selectively activated by 4-hydroxytamoxifen (4HT). 4HT stimulation did not have any effect on growth and morphology of M1/ STAT3ER cells, and did not induce the down-regulation of mRNA of c-myc and c-myb, which is necessary for M1 cell differentiation. On the other hand, mRNA of jun-B, IRF1 and p19 was increased by 4HT. DNA precipitation assay indicated that both stimulation of LIF and 4HT similarly activated STAT3ER. Introduction of a constitutive active MAP kinase kinase (MEK1) into M1/STAT3ER cells did not induce differentiation either. Together, our present data suggest that signaling other than the activation of STAT3 and MEK1 may be necessary for M1 cell-growth arrest and differentiation, while a set of early genes of LIF are induced by only STAT3 activation.
Journal Cover

February 2005
Volume 15 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.957
Ranked #26/121 Medicine Research and Experimental
(total number of cites)

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
APA
Yoshida, T., Iwamoto, T., Adachi, K., Yokota, T., Miyake, Y., & Hamaguchi, M. (2005). Functional analysis of the effect of forced activation of STAT3 on M1 mouse leukemia cells. International Journal of Molecular Medicine, 15(2), 269-275.
MLA
Yoshida, Iwamoto, Adachi, Yokota, Miyake, and Michinari Hamaguchi. "Functional analysis of the effect of forced activation of STAT3 on M1 mouse leukemia cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 15.2 (2005): 269-275.
Chicago
Yoshida, Iwamoto, Adachi, Yokota, Miyake, and Michinari Hamaguchi. "Functional analysis of the effect of forced activation of STAT3 on M1 mouse leukemia cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 15 no. 2 (2005): 269-275.