Sodium butyrate-mediated differentiation of colorectal cancer cells: Regulation of PKCβII by PI 3-kinase

  • Authors: Jolana Turecková, Martina Vojtechová, Dana Kucerová, Jiri Velek, Zdena Tuhácková
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  • Published online on: Tuesday, February 1, 2005
  • Pages: 329-335
  • DOI: 10.3892/ijmm.15.2.329

Abstract

The present study focuses on a putative regulation of PKCβII by phosphatidylinositol-3 kinase (PI 3-kinase) in colorectal carcinoma cells; little is known about the role and activity of PKCβII in these cells. We examined the activity of PI 3-kinase in two adenocarcinoma cell lines, HT29 cells that differentiate only after stimulation with appropriate agents, and Caco-2 cells that can differentiate spontaneously. The activity of PI 3-kinase as well as the activity of PKCβII appeared to decrease only in HT29 cells in which differentiation was induced by sodium butyrate. In HT29 cells infected with recombinant adenovirus encoding constitutively active PI 3-kinase, the activity of alkaline phosphatase was almost completely blocked, and this PI 3-kinase significantly potentiated the activity of PKCβII in HT29 cells despite the presence of NaBT in the culture medium. On the contrary, in differentiating Caco-2 cells, the activity of PI 3-kinase was not butyrate-sensitive. In agreement with these findings, the alkaline phosphatase activity was not affected by constitutively active PI 3-kinase overexpressed in Caco-2 cells. These observations suggest that PKCβII is regulated by PI 3-kinase in HT29 cells and that the mechanisms of spontaneous differentiation versus butyrate-induced differentiation of adenocarcinoma cells may be different.
Journal Cover

February 2005
Volume 15 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.88
Ranked #26/121 Medicine Research and Experimental
(total number of cites)

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APA
Turecková, J., Vojtechová, M., Kucerová, D., Velek, J., & Tuhácková, Z. (2005). Sodium butyrate-mediated differentiation of colorectal cancer cells: Regulation of PKCβII by PI 3-kinase. International Journal of Molecular Medicine, 15(2), 329-335.
MLA
Turecková, Vojtechová, Kucerová, Velek, and Zdena Tuhácková. "Sodium butyrate-mediated differentiation of colorectal cancer cells: Regulation of PKCβII by PI 3-kinase." International Journal of Molecular Medicine International Journal of Molecular Medicine 15.2 (2005): 329-335.
Chicago
Turecková, Vojtechová, Kucerová, Velek, and Zdena Tuhácková. "Sodium butyrate-mediated differentiation of colorectal cancer cells: Regulation of PKCβII by PI 3-kinase." International Journal of Molecular Medicine International Journal of Molecular Medicine 15 no. 2 (2005): 329-335.