Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection

  • Authors:
    • Lewis F. Neville
    • Yoav Barnea
    • Orly Hammer-Munz
    • Eyal Gur
    • Boris Kuzmenko
    • Hamutal Kahel-Raifer
    • Rachel Eren
    • Adi Elkeles
    • Kanneganti G.K. Murthy
    • Csaba Szabó
    • Andrew L. Salzman
    • Shlomo Dagan
    • Yehuda Carmeli
    • Shiri Navon-Venezia
  • View Affiliations

  • Published online on: Friday, July 1, 2005
  • Pages: 165-171
  • DOI: 10.3892/ijmm.16.1.165

Abstract

The goal of this study was to investigate if antibodies raised against N'-terminal Pseudomonas aeruginosa (Pa) flagellin could afford protection in two lethal mouse models of Pa infection. To that end, rabbit polyclonal antibodies were generated against the N'-terminal domains (amino acids 1-156) of recombinant Pa01 or Salmonella muenchen flagellins, termed anti-N'-fla-b and anti-N'-fla-Sm, respectively. In vitro, anti-N'-fla-b but not anti-N'-fla-Sm IgG specifically recognized recombinant and Pa endogenous flagellin type b proteins, total bacterial lysates of Pa type b, and inhibited Pa01 invasion into A549 cells. In vivo, administration of anti-N'-fla-b afforded a remarkable improvement in survival in lethal peritonitis (90% vs. 12% in control; p<0.001) and burn infection (83% vs. 8-17% in control groups; p<0.005) Pa models. These findings would suggest that the N'-terminal domain of Pa flagellin harbors critically important bioactive domains and that an antibody-targeted, neutralization approach directed at this region could provide a novel therapeutic strategy to combat Pa infection.
Journal Cover

July 2005
Volume 16 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Neville, L., Barnea, Y., Hammer-Munz, O., Gur, E., Kuzmenko, B., Kahel-Raifer, H., Eren, R., Elkeles, A., Murthy, K., Szabó, C., Salzman, A., Dagan, S., Carmeli, Y., & Navon-Venezia, S. (2005). Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection. International Journal of Molecular Medicine, 16(1), 165-171.
MLA
Neville, Barnea, Hammer-Munz, Gur, Kuzmenko, Kahel-Raifer, Eren, Elkeles, Murthy, Szabó, Salzman, Dagan, Carmeli, and Shiri Navon-Venezia. "Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection." International Journal of Molecular Medicine International Journal of Molecular Medicine 16.1 (2005): 165-171.
Chicago
Neville, Barnea, Hammer-Munz, Gur, Kuzmenko, Kahel-Raifer, Eren, Elkeles, Murthy, Szabó, Salzman, Dagan, Carmeli, and Shiri Navon-Venezia. "Antibodies raised against N'-terminal Pseudomonas aeruginosa flagellin prevent mortality in lethal murine models of infection." International Journal of Molecular Medicine International Journal of Molecular Medicine 16 no. 1 (2005): 165-171.