SDevelopment of dextran sulfate sodium-induced colitis is aggravated in mice genetically deficient for complement C5

  • Authors: Yasuyuki Deguchi, Akira Andoh, Osamu Inatomi, Yoshio Araki, Kazunori Hata, Tomoyuki Tsujikawa, Katsuyuki Kitoh, Yoshihide Fujiyama
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  • Published online on: Saturday, October 1, 2005
  • Pages: 605-608
  • DOI: 10.3892/ijmm.16.4.605

Abstract

The complement system is a potent effector of innate immunity. To elucidate the pathophysiological role of the complement system in inflammatory bowel disease (IBD), we evaluated the development of dextran sulfate sodium (DSS)-induced colitis in genetically complement C5-deficient mice. We used DBA2/J mice, which are genetically deficient in complement C5. DBA1/J mice have a normal complement system, and were used as controls. Experimental colitis was induced by the oral administration of 3.5% (w/v) DSS in their drinking water for 10 days. On day 10, all mice were sacrificed and their colons were collected. The development of colitis was assessed by the histological score, disease activity index, myeloperoxidase (MPO) activity, and macroscopic changes of the colon. Body weight loss was more apparent in the DBA2/J mice than in control DBA1/J mice. The colon length was shorter in the DBA2/J mice than in DBA1/J mice. The disease activity index, histological colitis score, and MPO activity were all significantly higher in the DBA2/J mice than in DBA1/J mice. Microscopically, mucosal edema, cellular infiltration and disruption of the epithelium were much more severe in the DBA2/J mice than in DBA1/J mice. The development of DSS colitis was aggravated in genetically C5-deficient DBA2/J mice. These findings suggest that the complement system might play a protective role in the development of DSS-induced experimental colitis.
Journal Cover

October 2005
Volume 16 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.957
Ranked #26/121 Medicine Research and Experimental
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APA
Deguchi, Y., Andoh, A., Inatomi, O., Araki, Y., Hata, K., Tsujikawa, T., Kitoh, K., & Fujiyama, Y. (2005). SDevelopment of dextran sulfate sodium-induced colitis is aggravated in mice genetically deficient for complement C5. International Journal of Molecular Medicine, 16(4), 605-608.
MLA
Deguchi, Andoh, Inatomi, Araki, Hata, Tsujikawa, Kitoh, and Yoshihide Fujiyama. "SDevelopment of dextran sulfate sodium-induced colitis is aggravated in mice genetically deficient for complement C5." International Journal of Molecular Medicine International Journal of Molecular Medicine 16.4 (2005): 605-608.
Chicago
Deguchi, Andoh, Inatomi, Araki, Hata, Tsujikawa, Kitoh, and Yoshihide Fujiyama. "SDevelopment of dextran sulfate sodium-induced colitis is aggravated in mice genetically deficient for complement C5." International Journal of Molecular Medicine International Journal of Molecular Medicine 16 no. 4 (2005): 605-608.