Systematic analysis of cDNA microarray-based CGH

  • Authors:
    • Chan Hee Park
    • Ha Jin Jeong
    • Yeon Ho Choi
    • Sang Cheol Kim
    • Hei Chul Jeong
    • Kyu Hyun Park
    • Gui Yeon Lee
    • Tae Soo Kim
    • Sang Wha Yang
    • Sung Whan Ahn
    • Yang Seok Kim
    • Sun Young Rha
    • Hyun Cheol Chung
  • View Affiliations

  • Published online on: Wednesday, February 1, 2006
  • Pages: 261-267
  • DOI: 10.3892/ijmm.17.2.261

Abstract

cDNA microarray-based CGH (Microarray-CGH) is a useful technique for detecting genomic aberrations with a high resolution. However, the criteria for determining a genomic alteration have not been determined. We evaluated the genome-wide measurement of copy number of each gene in normal gastric and placenta tissues with both sex-matched, direct and sex-mismatched, indirect designs using 17K cDNA microarray. The results revealed the range of genomic copy number of normal tissues to be ±0.3 of the log2 ratio (gain >0.3, loss <−0.3) in the autosomal genes with direct and indirect designs. The copy number at a gene level from the X chromosomal genes using the direct and indirect sex-mismatched designs was ±0.68 of the log2 ratio (amplification >0.68, deletion <−0.68). In summary, the suggested method can be used as a guideline for analysis of genomic aberration using a Microarray-CGH in both direct and indirect designs.
Journal Cover

February 2006
Volume 17 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Park, C., Jeong, H., Choi, Y., Kim, S., Jeong, H., Park, K., Lee, G., Kim, T., Yang, S., Ahn, S., Kim, Y., Rha, S., & Chung, H. (2006). Systematic analysis of cDNA microarray-based CGH. International Journal of Molecular Medicine, 17(2), 261-267.
MLA
Park, Jeong, Choi, Kim, Jeong, Park, Lee, Kim, Yang, Ahn, Kim, Rha, and Hyun Chung. "Systematic analysis of cDNA microarray-based CGH." International Journal of Molecular Medicine International Journal of Molecular Medicine 17.2 (2006): 261-267.
Chicago
Park, Jeong, Choi, Kim, Jeong, Park, Lee, Kim, Yang, Ahn, Kim, Rha, and Hyun Chung. "Systematic analysis of cDNA microarray-based CGH." International Journal of Molecular Medicine International Journal of Molecular Medicine 17 no. 2 (2006): 261-267.