Human membrane protein Tim-3 facilitates hepatitis A virus entry into target cells

  • Authors:
    • Lili Sui
    • Nan Li
    • Wenyuan Zhang
    • Yong Chen
    • Yuanyuan Zheng
    • Tao Wan
    • Weiping Zhang
    • Yadong Yang
    • Guojian Fang
    • Jiangsen Mao
    • Xuetao Cao
  • View Affiliations

  • Published online on: June 1, 2006     https://doi.org/10.3892/ijmm.17.6.1093
  • Pages: 1093-1099
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Abstract

In this study, a cellular surface membrane protein of immunoglobulin (Ig) superfamily (IgSF) was identified from a human dendritic cell (DC) cDNA library by large-scale random sequencing, which is identical to previously reported Tim-3 (T-cell Ig- and mucin-domain-containing molecule 3). Recent data have suggested the association of the 281-residue mouse Tim-3 molecule with Th1-related T cell responses and disease in mice. Human Tim-3 is a 301-residue type I membrane protein whose extracellular region contains a Cys-rich Ig-like domain and a mucin domain, the characteristics of Tim proteins. It shows significant homology to human hepatitis A virus (HAV) cellular receptor-1 (HuHAVcr-1)/Tim-1. Human Tim-3 mRNA was highly expressed in monocytes or monocyte-derived cells, and the expression level decreased when DC underwent maturation and activation. There is no previous report on the biological functions of human Tim-3, especially the involvement in virus infection. We demonstrated that HeLa cells, which are refractory to HAV infection, acquired a limited susceptibility to HAV infection after stably overexpressing human Tim-3 as confirmed by Western blot analysis using anti-Tim-3 antibody, but Tim-3-Fc fusion protein had no direct HAV-binding activity. The results indicated that human Tim-3 can promote HAV entry into target cells but itself may not function as a cellular receptor of HAV.

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June 2006
Volume 17 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Sui L, Li N, Zhang W, Chen Y, Zheng Y, Wan T, Zhang W, Yang Y, Fang G, Mao J, Mao J, et al: Human membrane protein Tim-3 facilitates hepatitis A virus entry into target cells. Int J Mol Med 17: 1093-1099, 2006.
APA
Sui, L., Li, N., Zhang, W., Chen, Y., Zheng, Y., Wan, T. ... Cao, X. (2006). Human membrane protein Tim-3 facilitates hepatitis A virus entry into target cells. International Journal of Molecular Medicine, 17, 1093-1099. https://doi.org/10.3892/ijmm.17.6.1093
MLA
Sui, L., Li, N., Zhang, W., Chen, Y., Zheng, Y., Wan, T., Zhang, W., Yang, Y., Fang, G., Mao, J., Cao, X."Human membrane protein Tim-3 facilitates hepatitis A virus entry into target cells". International Journal of Molecular Medicine 17.6 (2006): 1093-1099.
Chicago
Sui, L., Li, N., Zhang, W., Chen, Y., Zheng, Y., Wan, T., Zhang, W., Yang, Y., Fang, G., Mao, J., Cao, X."Human membrane protein Tim-3 facilitates hepatitis A virus entry into target cells". International Journal of Molecular Medicine 17, no. 6 (2006): 1093-1099. https://doi.org/10.3892/ijmm.17.6.1093