Mitochondria from the left heart ventricles of both normotensive and spontaneously hypertensive rats oxidize externally added NADH mostly via a novel malate/oxaloacetate shuttle as reconstructed in vitro

  • Authors:
    • Anna Atlante
    • Teresa M. Seccia
    • Lidia de Bari
    • Ersilia Marra
    • Salvatore Passarella
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/ijmm.18.1.177
  • Pages: 177-186
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Abstract

A substantial increase in NADH production, arising from accelerated glycolysis, occurs in cardiac hypertrophy and this raises the question of how the NADH is oxidised. We have addressed this problem by reconstructing appropriate mitochondrial shuttles in vitro, using mitochondria from the left ventricles of both normotensive and spontaneously hypertensive rats at 5 and 24 weeks of age as model systems for left ventricle hypertrophy and hypertrophy/hypertension respectively. We found that most NADH oxidation occurs via a novel malate/oxaloacetate shuttle, the activity of which increases with time and with the progression of hypertrophy and development of hypertension as judged by statistical ANOVA analysis. In contrast, α-glycerol-phosphate and the malate/aspartate shuttles were shown to make only a minor contribution to NADH oxidation in a manner essentially independent of age and progression of hypertrophy/hypertension. The rate of malate transport in exchange with oxaloacetate proved to limit the rate of NADH oxidation via this malate/oxaloacetate shuttle.

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July 2006
Volume 18 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Atlante A, Seccia TM, de Bari L, Marra E and Passarella S: Mitochondria from the left heart ventricles of both normotensive and spontaneously hypertensive rats oxidize externally added NADH mostly via a novel malate/oxaloacetate shuttle as reconstructed in vitro . Int J Mol Med 18: 177-186, 2006.
APA
Atlante, A., Seccia, T.M., de Bari, L., Marra, E., & Passarella, S. (2006). Mitochondria from the left heart ventricles of both normotensive and spontaneously hypertensive rats oxidize externally added NADH mostly via a novel malate/oxaloacetate shuttle as reconstructed in vitro . International Journal of Molecular Medicine, 18, 177-186. https://doi.org/10.3892/ijmm.18.1.177
MLA
Atlante, A., Seccia, T. M., de Bari, L., Marra, E., Passarella, S."Mitochondria from the left heart ventricles of both normotensive and spontaneously hypertensive rats oxidize externally added NADH mostly via a novel malate/oxaloacetate shuttle as reconstructed in vitro ". International Journal of Molecular Medicine 18.1 (2006): 177-186.
Chicago
Atlante, A., Seccia, T. M., de Bari, L., Marra, E., Passarella, S."Mitochondria from the left heart ventricles of both normotensive and spontaneously hypertensive rats oxidize externally added NADH mostly via a novel malate/oxaloacetate shuttle as reconstructed in vitro ". International Journal of Molecular Medicine 18, no. 1 (2006): 177-186. https://doi.org/10.3892/ijmm.18.1.177