Estradiol and resveratrol stimulating effect on osteocalcin, but not osteonectin and collagen-1α gene expression in primary culture of rat calvarial osteoblast-like cells

  • Authors:
    • Marcin Rucinski
    • Agnieszka Ziolkowska
    • Anna Hochol
    • Andrzej Pucher
    • Carlo Macchi
    • Anna S. Belloni
    • Gastone G. Nussdorfer
    • Ludwik K. Malendowicz
  • View Affiliations

  • Published online on: Sunday, October 1, 2006
  • Pages: 565-570
  • DOI: 10.3892/ijmm.18.4.565

Abstract

Evidence is available that some endocrine disruptors, acting as selective estrogen receptor modulators (SERMs), interfere with osteoblast differentiation and function. Therefore, we investigated whether 17β-estradiol, bisphenol-A (BSP), silymarin, genistein, resveratrol, procymidone, linurone and benzophenone-3 (BP3) modulate differentiation of rat calvarial osteoblast-like (ROB) cells in primary in vitro culture. Disruptors were added at day 18 of culture and cells were harvested 48 h later. Real time-PCR revealed that estradiol and resveratrol enhanced osteocalcin mRNA expression in ROB cells, while other disruptors were ineffective. The expression of osteonectin and collagen-1α was not affected by any disruptor. Estradiol, resveratrol, genistein and BSP stimulated the proliferative activity of ROB cells. In contrast, procymidone and linurone inhibited the proliferative activity, and silymarin and BP3 were ineffective. The conclusion is drawn that i) only resveratrol is able, like estradiol, to stimulate the specialized functions of ROB cells, and ii) the proliferative activity of ROB cells is more sensitive to endocrine disruptors, some of which could probably act via a mechanism independent of their SERM activity.
Journal Cover

October 2006
Volume 18 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
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APA
Rucinski, M., Ziolkowska, A., Hochol, A., Pucher, A., Macchi, C., Belloni, A., Nussdorfer, G., & Malendowicz, L. (2006). Estradiol and resveratrol stimulating effect on osteocalcin, but not osteonectin and collagen-1α gene expression in primary culture of rat calvarial osteoblast-like cells. International Journal of Molecular Medicine, 18(4), 565-570.
MLA
Rucinski, Ziolkowska, Hochol, Pucher, Macchi, Belloni, Nussdorfer, and Ludwik Malendowicz. "Estradiol and resveratrol stimulating effect on osteocalcin, but not osteonectin and collagen-1α gene expression in primary culture of rat calvarial osteoblast-like cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 18.4 (2006): 565-570.
Chicago
Rucinski, Ziolkowska, Hochol, Pucher, Macchi, Belloni, Nussdorfer, and Ludwik Malendowicz. "Estradiol and resveratrol stimulating effect on osteocalcin, but not osteonectin and collagen-1α gene expression in primary culture of rat calvarial osteoblast-like cells." International Journal of Molecular Medicine International Journal of Molecular Medicine 18 no. 4 (2006): 565-570.