Mitogen stimulation activates different signaling pathways in early- and late-divided T cells as revealed by DNA microarray analysis

  • Authors:
    • Yufeng Li
    • Kwong-Kwok Wong
    • Satoko Matsueda
    • Clay L. Efferson
    • David Z. Chang
    • Constantin G. Ioannides
    • Naotake Tsuda
  • View Affiliations

  • Published online on: December 1, 2006     https://doi.org/10.3892/ijmm.18.6.1127
  • Pages: 1127-1139
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Abstract

Mobilization of tumor-reactive CD8+ T cells remains the major challenge of cancer immunotherapy. Knowing how and when the T cell response expands and differentiates after antigen stimulation would make a significant contribution to the development of tumor vaccines. In the current study, we used CFSE-based cell sorting and cDNA microarray to identify the gene expression profile of adjacent generations of T cells after PHA stimulation. Early-divided generations of T cells responded to stimulation by activating cell cycle and surviving gene pathways, while late generations of T cells had more dramatic changes in transcription of cytokine genes. Reconstruction of biochemical pathways, activated in both early and late generations of T cells, also confirmed the impact of division in focal-adhesion kinases. Because most tumors are infiltrated by lymphocytes, our studies indicate a novel approach to identify ‘systemic biological responses’ of T cells, which could determine the design, and optimization of effective tumor vaccines.

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December 2006
Volume 18 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Li Y, Wong K, Matsueda S, Efferson CL, Chang DZ, Ioannides CG and Tsuda N: Mitogen stimulation activates different signaling pathways in early- and late-divided T cells as revealed by DNA microarray analysis. Int J Mol Med 18: 1127-1139, 2006.
APA
Li, Y., Wong, K., Matsueda, S., Efferson, C.L., Chang, D.Z., Ioannides, C.G., & Tsuda, N. (2006). Mitogen stimulation activates different signaling pathways in early- and late-divided T cells as revealed by DNA microarray analysis. International Journal of Molecular Medicine, 18, 1127-1139. https://doi.org/10.3892/ijmm.18.6.1127
MLA
Li, Y., Wong, K., Matsueda, S., Efferson, C. L., Chang, D. Z., Ioannides, C. G., Tsuda, N."Mitogen stimulation activates different signaling pathways in early- and late-divided T cells as revealed by DNA microarray analysis". International Journal of Molecular Medicine 18.6 (2006): 1127-1139.
Chicago
Li, Y., Wong, K., Matsueda, S., Efferson, C. L., Chang, D. Z., Ioannides, C. G., Tsuda, N."Mitogen stimulation activates different signaling pathways in early- and late-divided T cells as revealed by DNA microarray analysis". International Journal of Molecular Medicine 18, no. 6 (2006): 1127-1139. https://doi.org/10.3892/ijmm.18.6.1127