Reduced rate of adenosine triphosphate synthesis by in vivo 31P nuclear magnetic resonance spectroscopy and downregulation of PGC-1β in distal skeletal muscle following burn

  • Authors: A. Aria Tzika, Dionyssios Mintzopoulos, Katie Padfield, julie wilhelmy, Michael N. Mindrinos, Hongue Yu, Haihui Cao, Qunhao Zhang, Loukas G. Astrakas, Jiangwen Zhang, Yong-Ming Yu, Laurence G. Rahme, Ronald G. Tompkins
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  • Published online on: Friday, February 1, 2008
  • Pages: 201-208
  • DOI: 10.3892/ijmm.21.2.201

Abstract

Using a mouse model of burn trauma, we tested the hypothesis that severe burn trauma corresponding to 30% of total body surface area (TBSA) causes reduction in adenosine triphosphate (ATP) synthesis in distal skeletal muscle. We employed in vivo 31P nuclear magnetic resonance (NMR) in intact mice to assess the rate of ATP synthesis, and characterized the concomitant gene expression patterns in skeletal muscle in burned (30% TBSA) versus control mice. Our NMR results showed a significantly reduced rate of ATP synthesis and were complemented by genomic results showing downregulation of the ATP synthase mitochondrial F1F0 complex and PGC-1β gene expression. Our findings suggest that inflammation and muscle atrophy in burns are due to a reduced ATP synthesis rate that may be regulated upstream by PGC-1β. These findings implicate mitochondrial dysfunction in distal skeletal muscle following burn injury. That PGC-1β is a highly inducible factor in most tissues and responds to common calcium and cyclic adenosine monophosphate (cAMP) signaling pathways strongly suggests that it may be possible to develop drugs that can induce PGC-1β.
Journal Cover

February 2008
Volume 21 Issue 2

Print ISSN: 1107-3756
Online ISSN:1791-244X

2012 Impact Factor: 1.957
Ranked #26/121 Medicine Research and Experimental
(total number of cites)

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APA
Tzika, A., Mintzopoulos, D., Padfield, K., wilhelmy, j., Mindrinos, M., Yu, H., Cao, H., Zhang, Q., Astrakas, L., Zhang, J., Yu, Y., Rahme, L., & Tompkins, R. (2008). Reduced rate of adenosine triphosphate synthesis by in vivo 31P nuclear magnetic resonance spectroscopy and downregulation of PGC-1β in distal skeletal muscle following burn. International Journal of Molecular Medicine, 21(2), 201-208.
MLA
Tzika, Mintzopoulos, Padfield, wilhelmy, Mindrinos, Yu, Cao, Zhang, Astrakas, Zhang, Yu, Rahme, and Ronald Tompkins. "Reduced rate of adenosine triphosphate synthesis by in vivo 31P nuclear magnetic resonance spectroscopy and downregulation of PGC-1β in distal skeletal muscle following burn." International Journal of Molecular Medicine International Journal of Molecular Medicine 21.2 (2008): 201-208.
Chicago
Tzika, Mintzopoulos, Padfield, wilhelmy, Mindrinos, Yu, Cao, Zhang, Astrakas, Zhang, Yu, Rahme, and Ronald Tompkins. "Reduced rate of adenosine triphosphate synthesis by in vivo 31P nuclear magnetic resonance spectroscopy and downregulation of PGC-1β in distal skeletal muscle following burn." International Journal of Molecular Medicine International Journal of Molecular Medicine 21 no. 2 (2008): 201-208.