Murine intramyocellular lipids quantified by NMR act as metabolic biomarkers in burn trauma

  • Authors: A. Aria Tzika, Loukas G. Astrakas, Haihui Cao, Dionyssios Mintzopoulos, Qunhao Zhang, Katie Padfield, Hongue Yu, Michael N. Mindrinos, Laurence G. Rahme, Ronald G. Tompkins
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  • Published online on: Sunday, June 1, 2008
  • Pages: 825-832
  • DOI: 10.3892/ijmm.21.6.825

Abstract

It has been suggested that intramyocellular lipids (IMCLs) may serve as biomarkers of insulin resistance and mitochondrial dysfunction. Using a hind-limb mouse model of burn trauma, we tested the hypothesis that severe localized burn trauma involving 5% of the total body surface area causes a local increase in IMCLs in the leg skeletal muscle. We quantified IMCLs from ex vivo intact tissue specimens using High-Resolution Magic Angle Spinning (HRMAS) 1H NMR and characterized the accompanying gene expression patterns in burned versus control skeletal muscle specimens. We also quantified plasma-free fatty acids (FFAs) in burn versus control mice. Our results from HRMAS 1H NMR measurements indicated that IMCL levels were significantly increased in mice exposed to burn trauma. Furthermore, plasma FFA levels were also significantly increased, and gene expression of Glut4, insulin receptor substrate 1 (IRS1), glycolytic genes, and PGC-1β was downregulated in these mice. Backward stepwise multiple linear regression analysis demonstrated that IMCL levels correlated significantly with FFA levels, which were a significant predictor of IRS1 and PGC-1β gene expression. We conclude from these findings that IMCLs can serve as metabolic biomarkers in burn trauma and that FFAs and IMCLs may signal altered metabolic gene expression. This signaling may result in the observed burn-induced insulin resistance and skeletal muscle mitochondrial dysfunction. We believe that IMCLs may therefore be useful biomarkers in predicting the therapeutic effectiveness of hypolipidemic agents for patients with severe burns.
Journal Cover

June 2008
Volume 21 Issue 6

Print ISSN: 1107-3756
Online ISSN:1791-244X

2013 Impact Factor: 1.88
Ranked #27/122 Medicine Research and Experimental
(total number of cites)

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APA
Tzika, A., Astrakas, L., Cao, H., Mintzopoulos, D., Zhang, Q., Padfield, K., Yu, H., Mindrinos, M., Rahme, L., & Tompkins, R. (2008). Murine intramyocellular lipids quantified by NMR act as metabolic biomarkers in burn trauma. International Journal of Molecular Medicine, 21(6), 825-832.
MLA
Tzika, Astrakas, Cao, Mintzopoulos, Zhang, Padfield, Yu, Mindrinos, Rahme, and Ronald Tompkins. "Murine intramyocellular lipids quantified by NMR act as metabolic biomarkers in burn trauma." International Journal of Molecular Medicine International Journal of Molecular Medicine 21.6 (2008): 825-832.
Chicago
Tzika, Astrakas, Cao, Mintzopoulos, Zhang, Padfield, Yu, Mindrinos, Rahme, and Ronald Tompkins. "Murine intramyocellular lipids quantified by NMR act as metabolic biomarkers in burn trauma." International Journal of Molecular Medicine International Journal of Molecular Medicine 21 no. 6 (2008): 825-832.